{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T09:44:43Z","timestamp":1791020683057,"version":"4.1.0"},"reference-count":31,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1999,9,1]]},"abstract":"<jats:p> Corneal endothelial function is dependent on[Formula: see text] transport. However, the relative[Formula: see text] permeabilities of the apical and basolateral membranes are unknown. Using changes in intracellular pH secondary to removing CO<jats:sub>2<\/jats:sub>-[Formula: see text](at constant pH) or removing [Formula: see text]alone (at constant CO<jats:sub>2<\/jats:sub>) from apical or basolateral compartments, we determined the relative apical and basolateral [Formula: see text] permeabilities and their dependencies on Na<jats:sup>+<\/jats:sup> and Cl<jats:sup>\u2212<\/jats:sup>. Removal of CO<jats:sub>2<\/jats:sub>-[Formula: see text]from the apical side caused a steady-state alkalinization (+0.08 pH units), and removal from the basolateral side caused an acidification (\u22120.05 pH units). Removal of[Formula: see text] at constant CO<jats:sub>2<\/jats:sub> indicated that the basolateral[Formula: see text] fluxes were about three to four times the apical fluxes. Reducing perfusate Na<jats:sup>+<\/jats:sup> concentration to 10 mM had no effect on apical flux but slowed basolateral[Formula: see text] flux by one-half. In the absence of Cl<jats:sup>\u2212<\/jats:sup>, there was an apparent increase in apical [Formula: see text] flux under constant-pH conditions; however, no net change could be measured under constant-CO<jats:sub>2<\/jats:sub> conditions. Basolateral flux was slowed \u223c30% in the absence of Cl<jats:sup>\u2212<\/jats:sup>, but the net flux was unchanged. The steady-state alkalinization after removal of CO<jats:sub>2<\/jats:sub>-[Formula: see text]apically suggests that CO<jats:sub>2<\/jats:sub>diffusion may contribute to apical[Formula: see text] flux through the action of a membrane-associated carbonic anhydrase. Indeed, apical CO<jats:sub>2<\/jats:sub> fluxes were inhibited by the extracellular carbonic anhydrase inhibitor benzolamide and partially restored by exogenous carbonic anhydrase. The presence of membrane-bound carbonic anhydrase (CAIV) was confirmed by immunoblotting. We conclude that the Na<jats:sup>+<\/jats:sup>-dependent basolateral[Formula: see text] permeability is consistent with Na<jats:sup>+<\/jats:sup>-[Formula: see text]cotransport. Changes in[Formula: see text] flux in the absence of Cl<jats:sup>\u2212<\/jats:sup> are most likely due to Na<jats:sup>+<\/jats:sup>-[Formula: see text]cotransport-induced membrane potential changes that cannot be dissipated. Apical [Formula: see text] permeability is relatively low, but may be augmented by CO<jats:sub>2<\/jats:sub> diffusion in conjunction with a CAIV. <\/jats:p>","DOI":"10.1152\/ajpcell.1999.277.3.c545","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T22:10:19Z","timestamp":1514153419000},"page":"C545-C553","source":"Crossref","is-referenced-by-count":24,"title":["Apical and basolateral CO<sub>2<\/sub>-   HCO  3   \u2212    permeability  in cultured bovine corneal endothelial cells"],"prefix":"10.1152","volume":"277","author":[{"given":"Joseph A.","family":"Bonanno","sequence":"first","affiliation":[{"name":"School of Optometry, Indiana University, Bloomington, Indiana 47401; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Guan","sequence":"additional","affiliation":[{"name":"School of Optometry, University of California, Berkeley, California 94720"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey","family":"Jelamskii","sequence":"additional","affiliation":[{"name":"School of Optometry, Indiana University, Bloomington, Indiana 47401; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao Jun","family":"Kang","sequence":"additional","affiliation":[{"name":"School of Optometry, University of California, Berkeley, California 94720"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1990.70.1.79"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1997.0290"},{"key":"B3","first-page":"3058","volume":"33","author":"Bonanno J. 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