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The overall effect is an increase in both peak and steady-state whole cell currents induced by glutamate. Single-channel studies demonstrate a significant increase in open probability but no change in the mean single-channel open time or mean channel conductance. Reduction in oxygen levels increased and prolonged the PAPA-NO-induced change in both peak and steady-state glutamate currents in transfected HEK cells. PAPA-NO also enhanced cell death in primary cultures of rodent cortical neurons deprived of oxygen and glucose. This potentiation of neuronal injury was blocked by MK-801, indicating a critical involvement of NMDA receptor activation. The NO-induced increase in NMDA channel activity as well as NMDA receptor-mediated cell death provide firm evidence that NO modulates the NMDA channel in a manner consistent with both a physiological role under normoxic conditions and a pathophysiological role under hypoxic conditions.<\/jats:p>","DOI":"10.1152\/ajpcell.1999.277.4.c673","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T22:40:56Z","timestamp":1514155256000},"page":"C673-C683","source":"Crossref","is-referenced-by-count":39,"title":["Hypoxia modulates nitric oxide-induced regulation of NMDA receptor currents and neuronal cell death"],"prefix":"10.1152","volume":"277","author":[{"given":"Muyiwa","family":"Gbadegesin","sequence":"first","affiliation":[{"name":"Interdisciplinary Program in Neuroscience and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano","family":"Vicini","sequence":"additional","affiliation":[{"name":"Interdisciplinary Program in Neuroscience and"},{"name":"Department of Physiology and Biophysics, Georgetown University Medical Center, Washington, District of Columbia 20007;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra J.","family":"Hewett","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Connecticut Health Center, Farmington, Connecticut 06030; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"Wink","sequence":"additional","affiliation":[{"name":"Radiation Biology, National Cancer Institute, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Espey","sequence":"additional","affiliation":[{"name":"Radiation Biology, National Cancer Institute, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryszard M.","family":"Pluta","sequence":"additional","affiliation":[{"name":"Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carol A.","family":"Colton","sequence":"additional","affiliation":[{"name":"Interdisciplinary Program in Neuroscience and"},{"name":"Department of Physiology and Biophysics, Georgetown University Medical Center, Washington, District of Columbia 20007;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.51.32515"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(90)90343-E"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702295"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(95)00700-J"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(96)01147-X"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.29.5.1037"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1038\/347768a0"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81053-3"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(93)90088-C"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(88)90162-6"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.21.9741"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(08)60767-0"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(89)90093-2"},{"key":"B15","first-page":"561","volume":"30","author":"Constantine-Paton M.","year":"1994","journal-title":"Psychol. 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