{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T15:29:13Z","timestamp":1772897353595,"version":"3.50.1"},"reference-count":61,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1999,10,1]]},"abstract":"<jats:p> Cell swelling results in regulatory activation of multiple conductive anion pathways permeable toward a broad spectrum of intracellular organic osmolytes. Here, we explore the involvement of extracellular and intracellular Ca<jats:sup>2+<\/jats:sup> in volume-dependent [<jats:sup>3<\/jats:sup>H]taurine efflux from primary cultured astrocytes and compare the Ca<jats:sup>2+<\/jats:sup> sensitivity of this efflux in slow (high K<jats:sup>+<\/jats:sup> medium induced) and fast (hyposmotic medium induced) cell swelling. Neither Ca<jats:sup>2+<\/jats:sup>-free medium nor Ca<jats:sup>2+<\/jats:sup>-channel blockers prevented the volume-dependent [<jats:sup>3<\/jats:sup>H]taurine release. In contrast, loading cells with the membrane-permeable Ca<jats:sup>2+<\/jats:sup> chelator 1,2-bis(2-aminophenoxy)ethane- N, N, N\u2032, N\u2032-tetraacetic acid (BAPTA)-AM suppressed [<jats:sup>3<\/jats:sup>H]taurine efflux by 65\u201370% and 25\u201330% under high-K<jats:sup>+<\/jats:sup> and hyposmotic conditions, respectively. Fura 2 measurements confirmed that BAPTA-AM, but not Ca<jats:sup>2+<\/jats:sup>-free media, significantly reduced resting intracellular Ca<jats:sup>2+<\/jats:sup>concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>). The calmodulin antagonists trifluoperazine and fluphenazine reversibly and irreversibly, respectively, inhibited the high-K<jats:sup>+<\/jats:sup>-induced [<jats:sup>3<\/jats:sup>H]taurine release, consistent with their known actions on calmodulin. In hyposmotic conditions, the effects were less pronounced. These data suggest that volume-dependent taurine release requires minimal basal [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>and involves calmodulin-dependent step(s). Quantitative differences in Ca<jats:sup>2+<\/jats:sup>\/calmodulin sensitivity of high-K<jats:sup>+<\/jats:sup>-induced and hyposmotic medium-induced taurine efflux are due to both the effects of the inhibitors on high-K<jats:sup>+<\/jats:sup>-induced cell swelling and their effects on transport systems and\/or signaling mechanisms determining taurine efflux. <\/jats:p>","DOI":"10.1152\/ajpcell.1999.277.4.c823","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T22:40:56Z","timestamp":1514155256000},"page":"C823-C832","source":"Crossref","is-referenced-by-count":48,"title":["Volume-dependent taurine release from cultured  astrocytes requires permissive [Ca<sup>2+<\/sup>]<sub>i<\/sub>and calmodulin"],"prefix":"10.1152","volume":"277","author":[{"given":"Alexander A.","family":"Mongin","sequence":"first","affiliation":[{"name":"Division of Neurosurgery,"},{"name":"Neuroscience and Neuropharmacology Research Group, Albany Medical College, Albany, New York 12208"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaohui","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Neuroscience, and"},{"name":"Neuroscience and Neuropharmacology Research Group, Albany Medical College, Albany, New York 12208"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harold K.","family":"Kimelberg","sequence":"additional","affiliation":[{"name":"Division of Neurosurgery,"},{"name":"Department of Pharmacology and Neuroscience, and"},{"name":"Neuroscience and Neuropharmacology Research Group, Albany Medical College, Albany, New York 12208"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2740193"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1992.tb10064.x"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.14-07-04237.1994"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1038\/361315a0"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1975.tb06974.x"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1966.tb04267.x"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.463bt.x"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1007\/BF00968079"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83641-4"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1007\/BF00964777"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1985.65.1.101"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1983.sp014692"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1997.609bj.x"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1992.72.1.101"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1991.tb15669.x"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1995.83.6.1051"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(93)90824-7"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1997.68031088.x"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.10-05-01583.1990"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1995.51"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)43891-4"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1126\/science.6095454"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1002\/glia.440040504"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.10-02-00571.1990"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1021\/bi00455a012"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1992.72.4.1037"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-0186(97)00033-8"},{"key":"B28","first-page":"2013","volume":"24","author":"Mongin A. A.","year":"1998","journal-title":"Soc. Neurosci. Abstr."},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1999.276.5.C1226"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1007\/BF00967318"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a021466"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.13-06-02638.1993"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.264.2.C471"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490170316"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.273.3.C755"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1002\/glia.440030512"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1136(199707)20:3<254::AID-GLIA9>3.0.CO;2-7"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1002\/glia.440030514"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.266.1.C165"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.266.1.C172"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1002\/glia.440020105"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1007\/s002329900374"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1988.tb04844.x"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.274.6.C1511"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-24-07803.1996"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(76)90503-5"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490260205"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.41.030179.001111"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1007\/s002329900413"},{"key":"B50","first-page":"223","volume":"9","author":"Strange K.","year":"1994","journal-title":"News Physiol. Sci."},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1996.270.3.C711"},{"key":"B52","first-page":"1035","volume":"77","author":"Sugar I. P.","year":"1981","journal-title":"J. Physiol. Paris"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050023"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1038\/290527a0"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(93)90180-Y"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1996.270.5.C1511"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(91)90806-7"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1994.63031143.x"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1139\/y87-166"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(85)91156-4"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1997.69020594.x"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1999.277.4.C823","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:24:17Z","timestamp":1718652257000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1999.277.4.C823"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,10,1]]},"references-count":61,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1999,10,1]]}},"alternative-id":["10.1152\/ajpcell.1999.277.4.C823"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1999.277.4.c823","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,10,1]]}}}