{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T08:58:00Z","timestamp":1790499480810,"version":"4.1.0"},"reference-count":33,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1999,12,1]]},"abstract":"<jats:p> The purpose of the current experiments was 1) to assess basolateral Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-2Cl<jats:sup>\u2212<\/jats:sup>cotransporter (NKCC1) expression and 2) to ascertain the role of cystic fibrosis transmembrane conductance regulator (CFTR) in the regulation of this transporter in a prototypical pancreatic duct epithelial cell line. Previously validated human pancreatic duct cell lines (CFPAC-1), which exhibit physiological features prototypical of cystic fibrosis, and normal pancreatic duct epithelia (stable recombinant CFTR-bearing CFPAC-1 cells, termed CFPAC-WT) were grown to confluence before molecular and functional studies. High-stringency Northern blot hybridization, utilizing specific cDNA probes, confirmed that NKCC1 was expressed in both cell lines and its mRNA levels were twofold higher in CFPAC-WT cells than in CFPAC-1 cells ( P &lt; 0.01, n = 3). Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-2Cl<jats:sup>\u2212<\/jats:sup>cotransporter activity, assayed as the bumetanide-sensitive, Na<jats:sup>+<\/jats:sup>- and Cl<jats:sup>\u2212<\/jats:sup>-dependent[Formula: see text] entry into the cell (with[Formula: see text] acting as a substitute for K<jats:sup>+<\/jats:sup>), increased by \u223c115% in CFPAC-WT cells compared with CFPAC-1 cells ( P &lt; 0.01, n = 6). Reducing the intracellular Cl<jats:sup>\u2212<\/jats:sup> by incubating the cells in a Cl<jats:sup>\u2212<\/jats:sup>-free medium increased Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-2Cl<jats:sup>\u2212<\/jats:sup>cotransporter activity by twofold ( P&lt; 0.01, n = 4) only in CFPAC-WT cells. We concluded that NKCC1 is expressed in pancreatic duct cells and mediates the entry of Cl<jats:sup>\u2212<\/jats:sup>. NKCC1 activity is enhanced in the presence of an inward Cl<jats:sup>\u2212<\/jats:sup> gradient. The results further indicate that the presence of functional CFTR enhances the expression of NKCC1. We speculate that CFTR regulates this process in a Cl<jats:sup>\u2212<\/jats:sup>-dependent manner. <\/jats:p>","DOI":"10.1152\/ajpcell.1999.277.6.c1100","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T22:30:34Z","timestamp":1514154634000},"page":"C1100-C1110","source":"Crossref","is-referenced-by-count":38,"title":["CFTR upregulates the expression of the basolateral  Na<sup>+<\/sup>-K<sup>+<\/sup>-2Cl<sup>\u2212<\/sup>cotransporter in cultured pancreatic duct cells"],"prefix":"10.1152","volume":"277","author":[{"given":"Holli","family":"Shumaker","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Cincinnati, and Veterans Affairs Medical Center at Cincinnati, Cincinnati, Ohio 45267"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manoocher","family":"Soleimani","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Cincinnati, and Veterans Affairs Medical Center at Cincinnati, Cincinnati, Ohio 45267"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)31742-8"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI686"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1007\/s002329900190"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.31.19111"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.7.1991"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1097\/00006676-199810000-00004"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.46.28969"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(95)90325-9"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)47302-4"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90398-X"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)32499-7"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(97)00679-3"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1996.sp021582"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1996.sp021583"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.9.4725"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.6.3414"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115358"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1172\/JCI1042"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-6060(05)80317-4"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)40738-1"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9610(99)80109-3"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1007\/BF00373750"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1007\/BF01870212"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115695"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107223"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.10.4012"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1999.276.1.C16"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1007\/s002329900132"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.267.2.C375"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1995.269.4.F536"},{"key":"B36","first-page":"260","volume":"433","author":"Xinjun H.","year":"1997","journal-title":"Pfl\u00fcgers Arch."},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.6.2201"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1999.277.6.C1100","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:22:07Z","timestamp":1718652127000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1999.277.6.C1100"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,12,1]]},"references-count":33,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1999,12,1]]}},"alternative-id":["10.1152\/ajpcell.1999.277.6.C1100"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1999.277.6.c1100","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,12,1]]}}}