{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T18:16:52Z","timestamp":1789755412350,"version":"4.0.1"},"reference-count":37,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2000,1,1]]},"abstract":"<jats:p> Two estrogen receptor (ER) isoforms, ER\u03b1 and ER\u03b2, have been described. However, no information is available in any species regarding the comparison of ER\u03b1 and ER\u03b2 levels in pregnant intrauterine tissues. We investigated 1) distribution of ER\u03b1 and ER\u03b2 mRNA in myometrium, amnion, choriodecidua, and placenta; 2) their abundance in intrauterine tissues at term not in labor (NIL) and in spontaneous term labor (STL); and 3) immunolocalization of ER\u03b1 and ER\u03b2 in pregnant rhesus monkey myometrium. Myometrium, amnion, choriodecidua, and placenta were obtained at cesarean section from monkeys in STL at 156\u2013166 days gestational age (GA) ( n = 4) and from control monkeys NIL at 140\u2013152 days GA ( n = 4). RT-PCR was conducted to determine ER\u03b1 and ER\u03b2 and glyceraldehyde-3-phosphate dehydrogenase mRNA abundance in four intrauterine tissues of the pregnant rhesus monkey. The cloned ER\u03b2 PCR fragment was subjected to sequence analysis. ER\u03b1 and ER\u03b2 were localized in the myometrium by immunohistochemistry. We demonstrated that 1) rhesus monkey ER\u03b2 shares &gt;97% identity with human ER\u03b2 in the region sequenced; 2) both ERs were expressed in myometrium, amnion, and choriodecidua but not in placenta in the current study; 3) ER\u03b1 and ER\u03b2 were differentially distributed in myometrium and amnion; 4) ER\u03b1 and ER\u03b2 were immunolocalized in myometrial smooth cells and smooth muscle and endothelial cells of the myometrial blood vessels. The biological significance of these quantitative differences in ER subtypes merits further study. <\/jats:p>","DOI":"10.1152\/ajpcell.2000.278.1.c190","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T22:34:06Z","timestamp":1514154846000},"page":"C190-C198","source":"Crossref","is-referenced-by-count":20,"title":["Differential distribution of ER\u03b1 and ER\u03b2 mRNA  in intrauterine tissues of the pregnant rhesus monkey"],"prefix":"10.1152","volume":"278","author":[{"given":"Wen Xuan","family":"Wu","sequence":"first","affiliation":[{"name":"Laboratory for Pregnancy and Newborn Research, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao Hong","family":"Ma","sequence":"additional","affiliation":[{"name":"Laboratory for Pregnancy and Newborn Research, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon C. 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