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CCK induced NF-\u03baB nuclear translocation, and DNA binding was also blocked by GF-109203X and BAPTA. Direct activation of PKC with TPA induced mob-1 chemokine expression and activated NF-\u03baB DNA binding to a similar extent as did CCK. Increasing intracellular Ca<jats:sup>2+<\/jats:sup>using ionomycin had no effect on mob-1 mRNA levels or NF-\u03baB activity. Both CCK and TPA treatments decreased inhibitory \u03baB-\u03b1 (I\u03baB-\u03b1) levels, whereas ionomycin had no effect. However, the effects of TPA on I\u03baB-\u03b1 degradation were less complete than for CCK. In combination, TPA and ionomycin degraded I\u03baB-\u03b1 to a similar extent as CCK. Therefore, activation of NF-\u03baB and mob-1 expression by supraphysiological CCK is likely mediated by both PKC activation and elevated intracellular Ca<jats:sup>2+<\/jats:sup>. <\/jats:p>","DOI":"10.1152\/ajpcell.2000.278.2.c344","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T22:26:19Z","timestamp":1514154379000},"page":"C344-C351","source":"Crossref","is-referenced-by-count":83,"title":["CCK stimulates <i>mob<\/i>-1 expression and NF-\u03baB activation  via protein kinase C and intracellular Ca<sup>2+<\/sup>"],"prefix":"10.1152","volume":"278","author":[{"given":"Bing","family":"Han","sequence":"first","affiliation":[{"name":"Department of Physiology, University of Michigan Medical School, Ann Arbor, Michigan 48109-0622"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Craig D.","family":"Logsdon","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Michigan Medical School, Ann Arbor, Michigan 48109-0622"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81318-5"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.2.429"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.12.6607"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.51.32606"},{"key":"B5","doi-asserted-by":"crossref","first-page":"5264","DOI":"10.4049\/jimmunol.159.11.5264","volume":"159","author":"Bonizzi G.","year":"1997","journal-title":"J. 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