{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T03:39:01Z","timestamp":1773459541122,"version":"3.50.1"},"reference-count":42,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2000,7,1]]},"abstract":"<jats:p> The notion that intracellular Ca<jats:sup>2+<\/jats:sup> (Ca<jats:sub>i<\/jats:sub> <jats:sup>2+<\/jats:sup>) stores play a significant role in the chemoreception process in chemoreceptor cells of the carotid body (CB) appears in the literature in a recurrent manner. However, the structural identity of the Ca<jats:sup>2+<\/jats:sup> stores and their real significance in the function of chemoreceptor cells are unknown. To assess the functional significance of Ca<jats:sub>i<\/jats:sub> <jats:sup>2+<\/jats:sup> stores in chemoreceptor cells, we have monitored 1) the release of catecholamines (CA) from the cells using an in vitro preparation of intact rabbit CB and 2) the intracellular Ca<jats:sup>2+<\/jats:sup> concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) using isolated chemoreceptor cells; both parameters were measured in the absence or the presence of agents interfering with the storage of Ca<jats:sup>2+<\/jats:sup>. We found that threshold [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> for high extracellular K<jats:sup>+<\/jats:sup> (K<jats:sub>e<\/jats:sub> <jats:sup>+<\/jats:sup>) to elicit a release response is \u2248250 nM. Caffeine (10\u201340 mM), ryanodine (0.5 \u03bcM), thapsigargin (0.05\u20131 \u03bcM), and cyclopiazonic acid (10 \u03bcM) did not alter the basal or the stimulus (hypoxia, high K<jats:sub>e<\/jats:sub> <jats:sup>+<\/jats:sup>)-induced release of CA. The same agents produced Ca<jats:sub>i<\/jats:sub> <jats:sup>2+<\/jats:sup>transients of amplitude below secretory threshold; ryanodine (0.5 \u03bcM), thapsigargin (1 \u03bcM), and cyclopiazonic acid (10 \u03bcM) did not alter the magnitude or time course of the Ca<jats:sub>i<\/jats:sub> <jats:sup>2+<\/jats:sup>responses elicited by high K<jats:sub>e<\/jats:sub> <jats:sup>+<\/jats:sup>. Several potential activators of the phospholipase C system (bethanechol, ATP, and bradykinin), and thereby of inositol 1,4,5-trisphosphate receptors, produced minimal or no changes in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and did not affect the basal release of CA. It is concluded that, in the rabbit CB chemoreceptor cells, Ca<jats:sub>i<\/jats:sub> <jats:sup>2+<\/jats:sup> stores do not play a significant role in the instant-to-instant chemoreception process. <\/jats:p>","DOI":"10.1152\/ajpcell.2000.279.1.c51","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T22:52:46Z","timestamp":1514155966000},"page":"C51-C61","source":"Crossref","is-referenced-by-count":28,"title":["Intracellular Ca<sup>2+<\/sup> stores in chemoreceptor cells of the rabbit carotid body: significance for chemoreception"],"prefix":"10.1152","volume":"279","author":[{"given":"I.","family":"Vicario","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u0131\u0301mica y Biolog\u0131\u0301a Molecular y Fisiolog\u0131\u0301a, Instituto de Biolog\u00eda y Gen\u00e9tica Molecular and Facultad de Medicina, Universidad de Valladolid, 47005 Valladolid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Obeso","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u0131\u0301mica y Biolog\u0131\u0301a Molecular y Fisiolog\u0131\u0301a, Instituto de Biolog\u00eda y Gen\u00e9tica Molecular and Facultad de Medicina, Universidad de Valladolid, 47005 Valladolid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Rocher","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u0131\u0301mica y Biolog\u0131\u0301a Molecular y Fisiolog\u0131\u0301a, Instituto de Biolog\u00eda y Gen\u00e9tica Molecular and Facultad de Medicina, Universidad de Valladolid, 47005 Valladolid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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