{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T11:13:59Z","timestamp":1761218039175,"version":"3.41.0"},"reference-count":51,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2000,9,1]]},"abstract":"<jats:p>This study tested the hypothesis that the activity of the mitochondrial membrane permeability transition pore (PTP) affects the resting mitochondrial membrane potential (\u0394\u03a8) of normal, healthy cells and that the anti-apoptotic gene product Bcl-2 inhibits the basal activity of the PTP. \u0394\u03a8 was measured by both fluorometric and nonfluorometric methods with SY5Y human neuroblastoma cells and with GT1\u20137 hypothalamic cells and PC12 pheochromocytoma cells in the absence and presence of Bcl-2 gene overexpression. The resting \u0394\u03a8 of Bcl-2 nonexpressing PC12 and wild-type SY5Y cells was increased significantly by the presence of the PTP inhibitor cyclosporin A (CsA) or by intracellular Ca<jats:sup>2+<\/jats:sup>chelation through exposure to the acetoxymethyl ester of 1,2-bis(2-aminophenoxy)ethane- N,N,N\u2032,N\u2032-tetraacetic acid (BAPTA-AM). The \u0394\u03a8 of Bcl-2-overexpressing PC12 cells was larger than that of Bcl-2-negative cells and not significantly increased by CsA or by Ca<jats:sup>2+<\/jats:sup>chelation. CsA did not present a significant effect on the \u0394\u03a8 monitored in unstressed GT1\u20137 cells but did inhibit the decrease in \u0394\u03a8 elicited by the addition of t-butyl hydroperoxide, an oxidative inducer of the mitochondrial permeability transition. These results support the hypothesis that an endogenous PTP activity can contribute to lowering the basal \u0394\u03a8 of some cells and that Bcl-2 can regulate the endogenous activity of the mitochondrial PTP.<\/jats:p>","DOI":"10.1152\/ajpcell.2000.279.3.c852","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T22:44:07Z","timestamp":1514155447000},"page":"C852-C859","source":"Crossref","is-referenced-by-count":41,"title":["Elevation of resting mitochondrial membrane potential of neural cells by cyclosporin A, BAPTA-AM, and Bcl-2"],"prefix":"10.1152","volume":"279","author":[{"given":"Alicia J.","family":"Kowaltowski","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, The University of Maryland Baltimore, Baltimore, Maryland 21201;"},{"name":"Departamento de Patologia Cl\u0131\u0301nica, Faculdade de Ci\u00eancias M\u00e9dicas, Universidade Estadual de Campinas, Campinas, SP, Brazil;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soraya S.","family":"Smaili","sequence":"additional","affiliation":[{"name":"Section on Neuronal Secretory Systems, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892; and"},{"name":"Departamento de Farmacologia, Universidade Federal de S\u00e3o Paulo, UNIFESP, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James T.","family":"Russell","sequence":"additional","affiliation":[{"name":"Section on Neuronal Secretory Systems, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary","family":"Fiskum","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, The University of Maryland Baltimore, Baltimore, Maryland 21201;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1992.262.6.H1699"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4400565"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1992.tb10050.x"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510290521"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.8866"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(94)00166-H"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4165(99)00073-2"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4547(19980915)53:6<728::AID-JNR10>3.0.CO;2-U"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.142.4.975"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(94)90053-1"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(98)00177-7"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(89)82754-7"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)50352-5"},{"key":"B14","first-page":"3459","volume":"46","author":"Fiskum G","year":"1986","journal-title":"Cancer Res"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-14-05151.1998"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90430-1"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(98)00122-4"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(98)77920-2"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80301-3"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(98)00119-4"},{"key":"B21","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/S0022-3565(25)38135-8","volume":"265","author":"Imberti R","year":"1993","journal-title":"J Pharmacol Exp Ther"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(98)00084-X"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1007\/BF01868720"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1126\/science.8235659"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(96)00173-9"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5303.1132"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(98)00216-0"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(98)00112-1"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.26.12579"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.8.1261"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.18.9893"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1995.65062432.x"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80580-2"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.272.4.C1286"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1007\/BF02110644"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(99)77239-5"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1126\/science.8235595"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.17.4987"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(99)77214-0"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.4.1455"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.52.33493"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0173(97)00056-8"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1023\/A:1027344914565"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1054\/ceca.1999.0061"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.46.27510"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1084\/jem.184.4.1331"},{"key":"B49","first-page":"369","volume":"57","author":"Vercesi AE.","year":"1985","journal-title":"An Acad Bras Cienc"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.8661"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1002\/neu.480250307"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5303.1129"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4157(95)00003-A"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.2000.279.3.C852","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T05:24:45Z","timestamp":1751174685000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.2000.279.3.C852"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,9,1]]},"references-count":51,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2000,9,1]]}},"alternative-id":["10.1152\/ajpcell.2000.279.3.C852"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.2000.279.3.c852","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"type":"print","value":"0363-6143"},{"type":"electronic","value":"1522-1563"}],"subject":[],"published":{"date-parts":[[2000,9,1]]}}}