{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T18:46:19Z","timestamp":1759776379993},"reference-count":32,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2000,11,1]]},"abstract":"<jats:p> Two H<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-ATPase isoforms are present in kidney: the gastric, highly sensitive to Sch-28080, and the colonic, partially sensitive to ouabain. Upregulation of Sch-28080-sensitive H<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-ATPase, or \u201cgastric\u201d H<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-ATPase, has been demonstrated in hypokalemic rat inner medullary collecting duct cells (IMCDs). Nevertheless, only colonic H<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-ATPase mRNA and protein abundance increase in this condition. This study was designed to determine whether Sch-28080 inhibits transporters other than the gastric H<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-ATPase. In the presence of bumetanide, Sch-28080 (200 \u03bcM) and ouabain (2 mM) inhibited<jats:sup>86<\/jats:sup>Rb<jats:sup>+<\/jats:sup> uptake (&gt;90%). That<jats:sup>86<\/jats:sup>Rb<jats:sup>+<\/jats:sup> uptake was almost completely abolished by Sch-28080 indicates an effect of this agent on the Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-ATPase. ATPase assays in membranes, or lysed cells, demonstrated sensitivity to ouabain but not Sch-28080. Thus the inhibitory effect of Sch-28080 was dependent on cell integrity. <jats:sup>86<\/jats:sup>Rb<jats:sup>+<\/jats:sup>-uptake studies without bumetanide demonstrated that ouabain inhibited activity by only 50%. Addition of Sch-28080 (200 \u03bcM) blocked all residual activity. Intracellular ATP declined after Sch-28080 (200 \u03bcM) but recovered after removal of this agent. In conclusion, high concentrations of Sch-28080 inhibit K<jats:sup>+<\/jats:sup>-ATPase activity in mouse IMCD-3 (mIMCD-3) cells as a result of ATP depletion. <\/jats:p>","DOI":"10.1152\/ajpcell.2000.279.5.c1319","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T23:01:22Z","timestamp":1514156482000},"page":"C1319-C1326","source":"Crossref","is-referenced-by-count":33,"title":["Sch-28080 depletes intracellular ATP selectively  in mIMCD-3 cells"],"prefix":"10.1152","volume":"279","author":[{"given":"Juan","family":"Codina","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, University of Kansas School of Medicine, Kansas City, Kansas 66160-7350"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph","family":"Cardwell","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Kansas School of Medicine, Kansas City, Kansas 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