{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T17:20:15Z","timestamp":1767374415541},"reference-count":34,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2000,11,1]]},"abstract":"<jats:p> Effects of HCO<jats:sub>3<\/jats:sub> <jats:sup>\u2212<\/jats:sup> on protein kinase C (PKC)- and protein kinase A (PKA)-induced anion conductances were investigated in Necturus gallbladder epithelial cells. In HCO<jats:sub>3<\/jats:sub> <jats:sup>\u2212<\/jats:sup>-free media, activation of PKC via 12- O-tetradecanoylphorbol 13-acetate (TPA) depolarized apical membrane potential ( V <jats:sub>a<\/jats:sub>) and decreased fractional apical voltage ratio (F<jats:sub>R<\/jats:sub>). These effects were blocked by mucosal 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB), a Cl<jats:sup>\u2212<\/jats:sup> channel blocker. In HCO<jats:sub>3<\/jats:sub> <jats:sup>\u2212<\/jats:sup>media, TPA induced significantly greater changes in V <jats:sub>a<\/jats:sub> and F<jats:sub>R<\/jats:sub>. These effects were blocked only when NPPB was present in both mucosal and basolateral compartments. The data suggest that TPA activates NPPB-sensitive apical Cl<jats:sup>\u2212<\/jats:sup> conductance ( g <jats:sub>Cl<\/jats:sub> <jats:sup>a<\/jats:sup>) in the absence of HCO<jats:sub>3<\/jats:sub> <jats:sup>\u2212<\/jats:sup>; in its presence, TPA stimulated both NPPB-sensitive g <jats:sub>Cl<\/jats:sub> <jats:sup>a<\/jats:sup> and basolateral Cl<jats:sup>\u2212<\/jats:sup> conductance ( g <jats:sub>Cl<\/jats:sub> <jats:sup>b<\/jats:sup>). Activation of PKA via 3-isobutyl-1-methylxanthine (IBMX) also decreased V <jats:sub>a<\/jats:sub> and F<jats:sub>R<\/jats:sub>; however, these changes were not affected by external HCO<jats:sub>3<\/jats:sub> <jats:sup>\u2212<\/jats:sup>. We conclude that HCO<jats:sub>3<\/jats:sub> <jats:sup>\u2212<\/jats:sup> modulates the effects of PKC on g <jats:sub>Cl<\/jats:sub> <jats:sup>b<\/jats:sup>. In HCO<jats:sub>3<\/jats:sub> <jats:sup>\u2212<\/jats:sup> medium, TPA and IBMX also induced an initial transient hyperpolarization and increase in intracellular pH. Because these changes were independent of mucosal Na<jats:sup>+<\/jats:sup> and Cl<jats:sup>\u2212<\/jats:sup>, it is suggested that TPA and IBMX induce a transient increase in apical HCO<jats:sub>3<\/jats:sub> <jats:sup>\u2212<\/jats:sup>conductance. <\/jats:p>","DOI":"10.1152\/ajpcell.2000.279.5.c1385","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T23:01:22Z","timestamp":1514156482000},"page":"C1385-C1392","source":"Crossref","is-referenced-by-count":3,"title":["Effect of HCO<sub>3<\/sub> <sup>\u2212<\/sup> on TPA- and IBMX-induced anion conductances in <i>Necturus<\/i> gallbladder epithelial cells"],"prefix":"10.1152","volume":"279","author":[{"given":"Pamela","family":"Lyall","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, Indiana University School of Medicine, Indianapolis, Indiana 46202-5102; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"McD. Armstrong","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, Indiana University School of Medicine, Indianapolis, Indiana 46202-5102; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vijay","family":"Lyall","sequence":"additional","affiliation":[{"name":"Department of Physiology, Virginia Commonwealth University, Richmond, Virginia 23298-0551"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1985.249.5.C527"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1992.263.1.L1"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1987.253.6.C802"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1007\/BF01870649"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1985.248.5.C457"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.102.2.177"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1007\/BF02000617"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.266.2.C531"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1986.250.5.G691"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.19.7649"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1007\/BF01869462"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1007\/BF01870438"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1992.263.1.C187"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.103.1.1"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1021\/bi00316a032"},{"key":"B16","first-page":"A1237","volume":"6","author":"Lyall P","year":"1992","journal-title":"FASEB J"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1992.263.4.F722"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1994.266.5.F685"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/0300-9629(92)90201-Z"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.9.7.7737456"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.81.5.705"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1985.248.1.C58"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)42339-3"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1038\/305723a0"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.90.2.173"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1007\/BF01875426"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.85.3.409"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.53.030191.002045"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.49.030187.000343"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1007\/BF01870454"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1989.256.6.C1184"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1996.271.5.C1574"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1007\/BF00584942"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1007\/BF01871383"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.2000.279.5.C1385","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:05:18Z","timestamp":1718651118000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.2000.279.5.C1385"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,11,1]]},"references-count":34,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2000,11,1]]}},"alternative-id":["10.1152\/ajpcell.2000.279.5.C1385"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.2000.279.5.c1385","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,11,1]]}}}