{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T02:24:58Z","timestamp":1778293498700,"version":"3.51.4"},"reference-count":39,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2000,11,1]]},"abstract":"<jats:p> Heterologous expression of a variety of membrane proteins in Xenopus oocytes sometimes results in the appearance of a hyperpolarization-activated inward current. The nature of this current remains incompletely understood. Some investigators have suggested that this current is a Cl current, whereas others have identified it as a nonselective cation current. The purpose of this investigation was to characterize this current in more detail. The hyperpolarization-activated inward current ( I <jats:sub>IN<\/jats:sub>) present in native oocytes was composed of a current carried at least partly by Ca and Mg under physiological ionic conditions plus a Ca-activated Cl current. The Ca-activated Cl current was blocked by chelation of cytosolic Ca with 1,2-bis(2-aminophenoxy)ethane- N,N,N\u2032,N\u2032-tetraacetic acid. When Cl currents were blocked, the cation current could be carried by Ca, Mg, or Co, but not appreciably by Ba, Mn, or Cd. I <jats:sub>IN<\/jats:sub> was stimulated by intracellular acidification. The properties of I <jats:sub>IN<\/jats:sub> were quite different from those of the store-operated Ca current. Heterologous expression of transient receptor potential-like gene product (TRPL), one of the members of the transient receptor potential family of putative store-operated Ca channels, apparently resulted in alteration of the voltage sensitivity of the endogenous I <jats:sub>IN<\/jats:sub>. <\/jats:p>","DOI":"10.1152\/ajpcell.2000.279.5.c1401","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T23:01:22Z","timestamp":1514156482000},"page":"C1401-C1413","source":"Crossref","is-referenced-by-count":28,"title":["A hyperpolarization- and acid-activated nonselective cation current in <i>Xenopus<\/i> oocytes"],"prefix":"10.1152","volume":"279","author":[{"given":"Akinori","family":"Kuruma","sequence":"first","affiliation":[{"name":"Department of Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322-3030"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiyuki","family":"Hirayama","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322-3030"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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