{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:26:25Z","timestamp":1790861185467,"version":"4.1.0"},"reference-count":46,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2000,12,1]]},"abstract":"<jats:p>We investigated the influence of pregnancy on large-conductance calcium-activated potassium channel (BK<jats:sub>Ca<\/jats:sub>) activity ( NP<jats:sub>o<\/jats:sub>) and on channel expression in membranes of isolated human myometrial smooth muscle cells. NP<jats:sub>o<\/jats:sub>in inside-out patches was higher in pregnant myometria (PM) compared with nonpregnant myometria (NPM), and the half-maximal activation potential was shifted by 39 mV to more negative potentials. This effect was not due to an enhanced BK<jats:sub>Ca<\/jats:sub>channel expression. In the presence of cAMP kinase (PKA) or cGMP kinase (PKG), NP<jats:sub>o<\/jats:sub>increased in patches from PM but decreased in those from NPM. Western blot analysis and use of a specific PKG inhibitor (1 \u03bcM KT-5823) verified the existence of a partially active membrane-associated PKG. Inhibition of PKA by 100 nM PKI, the inhibitory peptide of PKA, had no effect on NP<jats:sub>o<\/jats:sub>. 8- p-Chlorophenylthio-cGMP (8-pCPT-cGMP) hyperpolarized cells from PM. This effect was abolished by iberiotoxin, a specific blocker of BK<jats:sub>Ca<\/jats:sub>channels. It is concluded that an endogenous, membrane-bound PKG in myometrial cells specifically enhances BK<jats:sub>Ca<\/jats:sub>channel activity during pregnancy and thus may contribute to uterine quiescence during pregnancy.<\/jats:p>","DOI":"10.1152\/ajpcell.2000.279.6.c1751","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T23:05:08Z","timestamp":1514156708000},"page":"C1751-C1759","source":"Crossref","is-referenced-by-count":68,"title":["BK<sub>Ca<\/sub>channel activation by membrane-associated cGMP kinase may contribute to uterine quiescence in pregnancy"],"prefix":"10.1152","volume":"279","author":[{"given":"Xiao-Bo","family":"Zhou","sequence":"first","affiliation":[{"name":"Abteilung Pharmakologie f\u00fcr Pharmazeuten and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge-Xin","family":"Wang","sequence":"additional","affiliation":[{"name":"Abteilung Pharmakologie f\u00fcr Pharmazeuten 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