{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T04:02:51Z","timestamp":1751256171462,"version":"3.41.0"},"reference-count":19,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2000,12,1]]},"abstract":"<jats:p>Rabbit renal proximal tubular transport of riboflavin (RF) was examined by using the in vitro isolated tubule perfusion technique. We found that proximal tubules actively reabsorbed ( J<jats:sub>lb<\/jats:sub>) and secreted ( J<jats:sub>bl<\/jats:sub>) RF. At 0.1 \u03bcM RF concentration, J<jats:sub>bl<\/jats:sub>was significantly higher than J<jats:sub>lb<\/jats:sub>, resulting in a net secretion. This net secretion of RF was decreased at 0.01 \u03bcM RF concentration and increased at 1 \u03bcM RF concentration. Both J<jats:sub>lb<\/jats:sub>and J<jats:sub>bl<\/jats:sub>were inhibited by lowering temperature or by adding iodoacetate, a metabolic inhibitor, and lumichrome, an RF analog, suggesting the involvement of carrier-mediated transport mechanisms. J<jats:sub>bl<\/jats:sub>was inhibited by probenecid, an anion transport inhibitor, and by para-aminohippuric acid, an organic anion, suggesting the relevance of RF secretion to renal organic anion transport. J<jats:sub>bl<\/jats:sub>was also inhibited by alkaline pH (8.0) and by the calmodulin inhibitor trifluoperazine, indicating the influence of pH and Ca<jats:sup>2+<\/jats:sup>\/calmodulin-dependent pathway on RF secretion. Finally, we found that addition of chlorpromazine, a phenothiazine derivative, inhibited both J<jats:sub>lb<\/jats:sub>and J<jats:sub>bl<\/jats:sub>, raising the concern about the nutritional status in patients receiving such a type of medication.<\/jats:p>","DOI":"10.1152\/ajpcell.2000.279.6.c1782","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T23:05:08Z","timestamp":1514156708000},"page":"C1782-C1786","source":"Crossref","is-referenced-by-count":6,"title":["Riboflavin transport by isolated perfused rabbit renal proximal tubules"],"prefix":"10.1152","volume":"279","author":[{"given":"Norimoto","family":"Yanagawa","sequence":"first","affiliation":[{"name":"Medical and Research Services, Sepulveda Veterans Affairs Medical Center, Sepulveda 91343;"},{"name":"Department of Medicine, School of Medicine, University of California at Los Angeles, Los Angeles 90024;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Remi N. 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