{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T09:00:42Z","timestamp":1791104442362,"version":"4.1.0"},"reference-count":54,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2000,12,1]]},"abstract":"<jats:p> In a series of experiments, cultured myotubes were exposed to passive stretch or pharmacological agents that block contractile activation. Under these experimental conditions, the formation of Z lines and A bands (morphological structures, resulting from the specific structural alignment of sarcomeric proteins, necessary for contraction) was assessed by immunofluorescence. The addition of an antagonist of the voltage-gated Na<jats:sup>+<\/jats:sup> channels [tetrodotoxin (TTX)] for 2 days in developing rat myotube cultures led to a nearly total absence of Z lines and A bands. When contractile activation was allowed to resume for 2 days, the Z lines and A bands reappeared in a significant way. The appearance of Z lines or A bands could not be inhibited nor facilitated by the application of a uniaxial passive stretch. Electrical stimulation of the cultures increased sarcomere assembly significantly. Antagonists of L-type Ca<jats:sup>2+<\/jats:sup> channels (verapamil, nifedipine) combined with electrical stimulation led to the absence of Z lines and A bands to the same degree as the TTX treatment. Western blot analysis did not show a major change in the amount of sarcomeric \u03b1-actinin nor a shift in myosin heavy chain phenotype as a result of a 2-day passive stretch or TTX treatment. Results of experiments suggest that temporal Ca<jats:sup>2+<\/jats:sup> transients play an important factor in the assembly and maintenance of sarcomeric structures during muscle fiber development. <\/jats:p>","DOI":"10.1152\/ajpcell.2000.279.6.c1801","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T23:05:08Z","timestamp":1514156708000},"page":"C1801-C1811","source":"Crossref","is-referenced-by-count":89,"title":["Formation of sarcomeres in developing myotubes: role of mechanical stretch and contractile activation"],"prefix":"10.1152","volume":"279","author":[{"given":"Patrick G.","family":"De Deyne","sequence":"first","affiliation":[{"name":"Department of Orthopedics, University of Maryland School of Medicine, Baltimore, Maryland 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