{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T07:52:12Z","timestamp":1767858732224,"version":"3.49.0"},"reference-count":25,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2000,12,1]]},"abstract":"<jats:p> The effect of extracellular acidification was tested on the native epithelial Na<jats:sup>+<\/jats:sup> channel (ENaC) in A6 epithelia and on the cloned ENaC expressed in Xenopusoocytes. Channel activity was determined utilizing blocker-induced fluctuation analysis in A6 epithelia and dual electrode voltage clamp in oocytes. In A6 cells, a decrease of extracellular pH (pH<jats:sub>o<\/jats:sub>) from 7.4 to 6.4 caused a slow stimulation of the amiloride-sensitive short-circuit current ( I <jats:sub>Na<\/jats:sub>) by 68.4 \u00b1 11% ( n = 9) at 60 min. This increase of I <jats:sub>Na<\/jats:sub> was attributed to an increase of open channel and total channel ( N <jats:sub>T<\/jats:sub>) densities. Similar changes were observed with pH<jats:sub>o<\/jats:sub> 5.4. The effects of pH<jats:sub>o<\/jats:sub> were blocked by buffering intracellular Ca<jats:sup>2+<\/jats:sup> with 5 \u03bcM 1,2-bis(2-aminophenoxy)ethane- N,N,N\u2032,N\u2032-tetraacetic acid. In oocytes, pH<jats:sub>o<\/jats:sub> 6.4 elicited a small transient increase of the slope conductance of the cloned ENaC (11.4 \u00b1 2.2% at 2 min) followed by a decrease to 83.7 \u00b1 11.7% of control at 60 min ( n = 6). Thus small decreases of pH<jats:sub>o<\/jats:sub>stimulate the native ENaC by increasing N <jats:sub>T<\/jats:sub> but do not appreciably affect ENaC expressed in Xenopus oocytes. These effects are distinct from those observed with decreasing intracellular pH with permeant buffers that are known to inhibit ENaC. <\/jats:p>","DOI":"10.1152\/ajpcell.2000.279.6.c1896","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T23:05:08Z","timestamp":1514156708000},"page":"C1896-C1905","source":"Crossref","is-referenced-by-count":28,"title":["Regulation of the epithelial Na<sup>+<\/sup> channel by extracellular acidification"],"prefix":"10.1152","volume":"279","author":[{"given":"Mouhamed S.","family":"Awayda","sequence":"first","affiliation":[{"name":"Departments of Medicine and of Physiology, Tulane University School of Medicine, New Orleans, Louisiana 70112"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Boudreaux","sequence":"additional","affiliation":[{"name":"Departments of Medicine and of Physiology, Tulane University School of Medicine, New Orleans, Louisiana 70112"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roxanne L.","family":"Reger","sequence":"additional","affiliation":[{"name":"Departments of Medicine and of Physiology, Tulane University School of Medicine, New Orleans, Louisiana 70112"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. 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