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Similarly, spermine NONOate, a nitric oxide donor, increased K<jats:sub>Ca<\/jats:sub>currents (20 \u00b1 4% at 60 mV) in oocytes expressing the channel subunits alone. Stimulation of K<jats:sub>Ca<\/jats:sub>currents by ANP was inhibited in a concentration-dependent manner by a peptide inhibitor of cGMP-dependent protein kinase (PKG). Receptor\/channel stimulatory coupling was not completely abolished by mutating the cAMP-dependent protein kinase phosphorylation site on the \u03b1-subunit (S869; Nars M, Dhulipals PD, Wang YX, and Kotlikoff MI. J Biol Chem 273: 14920\u201314924, 1998) or by mutating a neighboring consensus PKG site (S855), but mutation of both residues virtually abolished coupling. Spermine NONOate also failed to stimulate channels expressed from the double mutant cRNAs. These data indicate that nitric oxide donors stimulate K<jats:sub>Ca<\/jats:sub>channels through cGMP-dependent phosphorylation and that two serine residues (855 and 869) underlie this stimulatory coupling.<\/jats:p>","DOI":"10.1152\/ajpcell.2000.279.6.c1938","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T23:05:08Z","timestamp":1514156708000},"page":"C1938-C1945","source":"Crossref","is-referenced-by-count":43,"title":["Guanylyl cyclase stimulatory coupling to K<sub>Ca<\/sub>channels"],"prefix":"10.1152","volume":"279","author":[{"given":"M.","family":"Nara","sequence":"first","affiliation":[{"name":"Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6046; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. D. 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