{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T01:38:21Z","timestamp":1770341901203,"version":"3.49.0"},"reference-count":30,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2001,2,1]]},"abstract":"<jats:p> Isolated single smooth muscle cells (SMCs) from different regions of the rabbit stomach were used to determine a possible correlation between unloaded shortening velocity and smooth muscle (SM) myosin heavy chain (MHC) S1 head isoform composition (SMA, no head insert; SMB, with head insert). \u03b1-Toxin-permeabilized isolated single cells were maximally activated to measure unloaded shortening velocity and subsequently used in an RT-PCR reaction to determine the SMA\/SMB content of the same cell. SM MHC SMA and SMB isoforms are uniquely distributed in the stomach with cells from the fundic region expressing little SMB (38.1 \u00b1 7.3% SMB; n = 16); cells from the antrum express primarily SMB (94.9 \u00b1 1.0% SMB; n = 16). Mean fundic cell unloaded shortening velocity was 0.014 \u00b1 0.002 cell lengths\/s compared with 0.036 \u00b1 0.002 for the antrum cells. Unloaded shortening velocity in these cells was significantly correlated with their percent SMB expression ( r <jats:sup>2<\/jats:sup> = 0.58). Resting cell length does not correlate with the percent SMB expression ( n = 32 cells). Previously published assays of purified or expressed SMA and SMB heavy meromyosin show a twofold difference in actin filament sliding speed in in vitro motility assays. Extrapolation of our data to 0\u2013100% SMB would give a 10-fold range of shortening velocity, which is closer to the \u223c20-fold range reported from various SM tissues. 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