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Three pharmacologically separable K<jats:sup>+<\/jats:sup> currents had properties of Kv1.3 and the Ca<jats:sup>2+<\/jats:sup>\/calmodulin-gated channels, SK2, SK3, and SK4. mRNA was detected for Kv1.3, Kv1.5, SK2, and\/or SK3, and SK4. Protein was detected for Kv1.3, Kv1.5, and SK3 (selective SK2 and SK4 antibodies not available). No Kv1.5-like current was detected, and confocal immunofluorescence showed the protein to be subcellular, in contrast to the robust membrane localization of Kv1.3. To determine whether any of these channels play a role in microglial activation, a respiratory burst was stimulated with phorbol 12-myristate 13-acetate and measured using a single cell, fluorescence-based dihydrorhodamine 123 assay. The respiratory burst was markedly inhibited by blockers of SK2 (apamin) and SK4 channels (clotrimazole and charybdotoxin), and to a lesser extent, by the potent Kv1.3 blocker agitoxin-2. <\/jats:p>","DOI":"10.1152\/ajpcell.2001.280.4.c796","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T20:32:16Z","timestamp":1514147536000},"page":"C796-C806","source":"Crossref","is-referenced-by-count":150,"title":["K<sup>+<\/sup> channels and the microglial respiratory burst"],"prefix":"10.1152","volume":"280","author":[{"given":"Rajesh","family":"Khanna","sequence":"first","affiliation":[{"name":"Division of Cellular and Molecular Biology, Toronto Western Research Institute, University Health Network, and"},{"name":"Department of Physiology, University of Toronto, Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lipi","family":"Roy","sequence":"additional","affiliation":[{"name":"Division of Cellular and Molecular 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