{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T21:55:48Z","timestamp":1771538148355,"version":"3.50.1"},"reference-count":30,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2001,4,1]]},"abstract":"<jats:p>Rat sublingual gland M<jats:sub>1<\/jats:sub>and M<jats:sub>3<\/jats:sub>muscarinic receptors each directly activate exocrine secretion. To investigate the functional role of coreceptor expression, we determined receptor-G protein coupling. Although membrane proteins of 40 and 41 kDa are ADP-ribosylated by pertussis toxin (PTX), and 44 kDa proteins by cholera toxin (CTX), both carbachol-stimulated high-affinity GTPase activity and the GTP-induced shift in agonist binding are insensitive to CTX or PTX. Carbachol enhances photoaffinity labeling ([\u03b1-<jats:sup>32<\/jats:sup>P]GTP-azidoaniline) of only 42-kDa proteins that are subsequently tractable to immunoprecipitation by antibodies specific for G\u03b1<jats:sub>q<\/jats:sub>or G\u03b1<jats:sub>11<\/jats:sub>but not G\u03b1<jats:sub>12<\/jats:sub>or G\u03b1<jats:sub>13<\/jats:sub>. Carbachol-stimulated photoaffinity labeling as well as phosphatidylinositol 4,5-bisphosphate (PIP<jats:sub>2<\/jats:sub>) hydrolysis is reduced 55% and 60%, respectively, by M<jats:sub>1<\/jats:sub>receptor blockade with m1-toxin. G\u03b1<jats:sub>q\/11<\/jats:sub>-specific antibody blocks carbachol-stimulated PIP<jats:sub>2<\/jats:sub>hydrolysis. We also provide estimates of the molar ratios of receptors to G\u03b1<jats:sub>q<\/jats:sub>and G\u03b1<jats:sub>11<\/jats:sub>. Although simultaneous activation of M<jats:sub>1<\/jats:sub>and M<jats:sub>3<\/jats:sub>receptors is required for a maximal response, both receptor subtypes are coupled to G\u03b1<jats:sub>q<\/jats:sub>and G\u03b1<jats:sub>11<\/jats:sub>to stimulate exocrine secretion via redundant mechanisms.<\/jats:p>","DOI":"10.1152\/ajpcell.2001.280.4.c884","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T20:32:16Z","timestamp":1514147536000},"page":"C884-C896","source":"Crossref","is-referenced-by-count":27,"title":["G protein coupling to M<sub>1<\/sub>and M<sub>3<\/sub>muscarinic receptors in sublingual glands"],"prefix":"10.1152","volume":"280","author":[{"given":"W.","family":"Luo","sequence":"first","affiliation":[{"name":"Center for Oral Biology and the Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York 14642"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. 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