{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T15:41:10Z","timestamp":1785771670570,"version":"3.56.0"},"reference-count":48,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2001,5,1]]},"abstract":"<jats:p>To characterize Ca<jats:sup>2+<\/jats:sup>transport in newborn rat cortical collecting duct (CCD) cells, we used nifedipine, which in adult rat distal tubules inhibits the intracellular Ca<jats:sup>2+<\/jats:sup>concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) increase in response to hormonal activation. We found that the dihydropyridine (DHP) nifedipine (20 \u03bcM) produced an increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>from 87.6 \u00b1 3.3 nM to 389.9 \u00b1 29.0 nM in 65% of the cells. Similar effects of other DHP (BAY K 8644, isradipine) were also observed. Conversely, DHPs did not induce any increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>in cells obtained from proximal convoluted tubule. In CCD cells, neither verapamil nor diltiazem induced any rise in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>. Experiments in the presence of EGTA showed that external Ca<jats:sup>2+<\/jats:sup>was required for the nifedipine effect, while lanthanum (20 \u03bcM), gadolinium (100 \u03bcM), and diltiazem (20 \u03bcM) inhibited the effect. Experiments done in the presence of valinomycin resulted in the same nifedipine effect, showing that K<jats:sup>+<\/jats:sup>channels were not involved in the nifedipine-induced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>rise. H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>also triggered [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>rise. However, nifedipine-induced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>increase was not affected by protamine. In conclusion, the present results indicate that 1) primary cultures of cells from terminal nephron of newborn rats are a useful tool for investigating Ca<jats:sup>2+<\/jats:sup>transport mechanisms during growth, and 2) newborn rat CCD cells in primary culture exhibit a new apical nifedipine-activated Ca<jats:sup>2+<\/jats:sup>channel of capacitive type (either transient receptor potential or leak channel).<\/jats:p>","DOI":"10.1152\/ajpcell.2001.280.5.c1193","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T20:22:34Z","timestamp":1514146954000},"page":"C1193-C1203","source":"Crossref","is-referenced-by-count":6,"title":["Nifedipine-activated Ca<sup>2+<\/sup>permeability in newborn rat cortical collecting duct cells in primary culture"],"prefix":"10.1152","volume":"280","author":[{"given":"Laura","family":"Valencia","sequence":"first","affiliation":[{"name":"Departamento de Fisiolog\u0131\u0301a, Centro de Investigaci\u00f3n y de Estudios 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