{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T09:44:56Z","timestamp":1770284696954,"version":"3.49.0"},"reference-count":32,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2001,6,1]]},"abstract":"<jats:p>The possible existence of transepithelial bicarbonate transport across the isolated bovine ciliary body was investigated by employing a chamber that allows for the measurement of unidirectional, radiolabeled fluxes of CO<jats:sub>2<\/jats:sub>+ HCO[Formula: see text]. No net flux of HCO[Formula: see text] was detected. However, acetazolamide (0.1 mM) reduced the simultaneously measured short-circuit current ( I<jats:sub>sc<\/jats:sub>). In other experiments in which<jats:sup>36<\/jats:sup>Cl<jats:sup>\u2212<\/jats:sup>was used, a net Cl<jats:sup>\u2212<\/jats:sup>flux of 1.12 \u03bceq \u00b7 h<jats:sup>\u22121<\/jats:sup>\u00b7 cm<jats:sup>\u22122<\/jats:sup>(30 \u03bcA\/cm<jats:sup>2<\/jats:sup>) in the blood-to-aqueous direction was detected. Acetazolamide, as well as removal of HCO[Formula: see text] from the aqueous bathing solution, inhibited the net Cl<jats:sup>\u2212<\/jats:sup>flux and I<jats:sub>sc<\/jats:sub>. Because such removal should increase HCO[Formula: see text] diffusion toward the aqueous compartment and increase the I<jats:sub>sc<\/jats:sub>, this paradoxical effect could result from cell acidification and partial closure of Cl<jats:sup>\u2212<\/jats:sup>channels. The acetazolamide effect on Cl<jats:sup>\u2212<\/jats:sup>fluxes can be explained by a reduction of cellular H<jats:sup>+<\/jats:sup>and HCO[Formula: see text] (generated from metabolic CO<jats:sub>2<\/jats:sub>production), which exchange with Na<jats:sup>+<\/jats:sup>and Cl<jats:sup>\u2212<\/jats:sup>via Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup>and Cl<jats:sup>\u2212<\/jats:sup>\/HCO[Formula: see text] exchangers, contributing to the net Cl<jats:sup>\u2212<\/jats:sup>transport. The fact that the net Cl<jats:sup>\u2212<\/jats:sup>flux is about three times larger than the I<jats:sub>sc<\/jats:sub>is explained with a vectorial model in which there is a secretion of Na<jats:sup>+<\/jats:sup>and K<jats:sup>+<\/jats:sup>into the aqueous humor that partially subtracts from the net Cl<jats:sup>\u2212<\/jats:sup>flux. These transport characteristics of the bovine ciliary epithelium suggest how acetazolamide reduces intraocular pressure in the absence of HCO[Formula: see text] transport as a driving force for fluid secretion.<\/jats:p>","DOI":"10.1152\/ajpcell.2001.280.6.c1521","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T20:14:14Z","timestamp":1514146454000},"page":"C1521-C1530","source":"Crossref","is-referenced-by-count":29,"title":["Model of ionic transport for bovine ciliary epithelium: effects of acetazolamide and HCO 3 \u2212"],"prefix":"10.1152","volume":"280","author":[{"given":"Chi-Ho","family":"To","sequence":"first","affiliation":[{"name":"Laboratory of Ocular Physiology and Biochemistry, Department of Optometry and Radiography, Hong Kong Polytechnic University, Hung Hom, Hong Kong; and Departments of"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi-Wai","family":"Do","sequence":"additional","affiliation":[{"name":"Laboratory of Ocular Physiology and Biochemistry, Department of Optometry and Radiography, Hong Kong Polytechnic University, Hung Hom, Hong Kong; 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