{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T15:51:17Z","timestamp":1790351477605,"version":"4.1.0"},"reference-count":37,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2001,6,1]]},"abstract":"<jats:p>Extracellular nucleotides cause elevation of cytosolic free Ca<jats:sup>2+<\/jats:sup>concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) in osteoclasts, although the sources of Ca<jats:sup>2+<\/jats:sup>are uncertain. Activation of P2Y receptors causes Ca<jats:sup>2+<\/jats:sup>release from stores, whereas P2X receptors are ligand-gated channels that mediate Ca<jats:sup>2+<\/jats:sup>influx in some cell types. To examine the sources of Ca<jats:sup>2+<\/jats:sup>, we studied osteoclasts from rat and rabbit using fura 2 fluorescence and patch clamp. Nucleotide-induced rise of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>persisted on removal of extracellular Ca<jats:sup>2+<\/jats:sup>(Ca[Formula: see text]), indicating involvement of stores. Inhibition of phospholipase C (PLC) with U-73122 or inhibition of endoplasmic reticulum Ca<jats:sup>2+<\/jats:sup>-ATPase with cyclopiazonic acid or thapsigargin abolished the rise of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>. After store depletion in the absence of Ca[Formula: see text], addition of Ca[Formula: see text] led to a rise of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>consistent with store-operated Ca<jats:sup>2+<\/jats:sup>influx. Store-operated Ca<jats:sup>2+<\/jats:sup>influx was greater at negative potentials and was blocked by La<jats:sup>3+<\/jats:sup>. In patch-clamp studies where PLC was blocked, ATP induced inward current indicating activation of P2X<jats:sub>4<\/jats:sub>nucleotide receptors, but with no rise of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>. We conclude that nucleotide-induced elevation of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>in osteoclasts arises primarily through activation of P2Y nucleotide receptors, leading to release of Ca<jats:sup>2+<\/jats:sup>from intracellular stores.<\/jats:p>","DOI":"10.1152\/ajpcell.2001.280.6.c1531","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T20:14:14Z","timestamp":1514146454000},"page":"C1531-C1539","source":"Crossref","is-referenced-by-count":32,"title":["Activation of P2Y but not P2X<sub>4<\/sub>nucleotide receptors causes elevation of [Ca<sup>2+<\/sup>]<sub>i<\/sub>in mammalian osteoclasts"],"prefix":"10.1152","volume":"280","author":[{"given":"A. 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