{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T10:36:33Z","timestamp":1791023793283,"version":"4.1.0"},"reference-count":36,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2001,6,1]]},"abstract":"<jats:p> Lack of functional calpain 3 in humans is a cause of limb girdle muscular dystrophy, but the function(s) of calpain 3 remain(s) unknown. Special muscle conditions in which calpain 3 is downregulated could yield valuable clues to the understanding of its function(s). We monitored calpain 3 mRNA amounts by quantitative RT-PCR and compared them with those of \u03b1-skeletal actin mRNA in mouse leg muscles for different types of denervation and muscle injury. Intact muscle denervation reduced calpain 3 mRNA expression by a factor of 5 to 10, while \u03b1-skeletal actin mRNA was reduced in a slower and less extensive manner. Muscle injury (denervation-devascularization), which leads to muscle degeneration and regeneration, induced a 20-fold decrease in the mRNA level of both calpain 3 and \u03b1-skeletal actin. Furthermore, whereas in normal muscle and intact denervated muscle, the full-length transcript is the major calpain 3 mRNA, in injured muscle, isoforms lacking exon 6 are predominant during the early regeneration process. These data suggest that muscle condition determines the specific calpain 3 isoform pattern of expression and that calpain 3 expression is downregulated by denervation. <\/jats:p>","DOI":"10.1152\/ajpcell.2001.280.6.c1561","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T20:14:14Z","timestamp":1514146454000},"page":"C1561-C1569","source":"Crossref","is-referenced-by-count":43,"title":["Calpain 3 mRNA expression in mice after denervation and during muscle regeneration"],"prefix":"10.1152","volume":"280","author":[{"given":"Daniel","family":"Stockholm","sequence":"first","affiliation":[{"name":"G\u00e9n\u00e9thon, CNRS URA 1922-1923, 91002 Evry;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muriel","family":"Herasse","sequence":"additional","affiliation":[{"name":"G\u00e9n\u00e9thon, CNRS URA 1922-1923, 91002 Evry;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Marchand","sequence":"additional","affiliation":[{"name":"G\u00e9n\u00e9thon, CNRS URA 1922-1923, 91002 Evry;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Praud","sequence":"additional","affiliation":[{"name":"Laboratoire de Physiologie, Atelier de R\u00e9g\u00e9n\u00e9ration Neuromusculaire, Facult\u00e9 de M\u00e9decine Saint-Antoine, 75012 Paris, France; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carinne","family":"Roudaut","sequence":"additional","affiliation":[{"name":"G\u00e9n\u00e9thon, CNRS URA 1922-1923, 91002 Evry;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Richard","sequence":"additional","affiliation":[{"name":"G\u00e9n\u00e9thon, CNRS URA 1922-1923, 91002 Evry;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Sebille","sequence":"additional","affiliation":[{"name":"Laboratoire de Physiologie, Atelier de R\u00e9g\u00e9n\u00e9ration Neuromusculaire, Facult\u00e9 de M\u00e9decine Saint-Antoine, 75012 Paris, France; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques S.","family":"Beckmann","sequence":"additional","affiliation":[{"name":"G\u00e9n\u00e9thon, CNRS URA 1922-1923, 91002 Evry;"},{"name":"Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.880140311"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1139\/o98-007"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1038\/10579"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19981123)78:5<661::AID-IJC22>3.0.CO;2-I"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(20000301)258:3<305::AID-AR10>3.0.CO;2-A"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4165(00)00050-7"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.8378"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(76)90007-8"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(83)90382-5"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/9.2.165"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1997.5160"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)38923-9"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.3.2005"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1101\/gr.6.10.986"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.6.4047"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1139\/o98-107"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(88)90048-7"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(93)90212-8"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(93)81468-F"},{"key":"B20","first-page":"169","volume":"40","author":"Laurent A","year":"1997","journal-title":"Ann Genet"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/0960-8966(95)00012-C"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1101\/gr.4.6.357"},{"key":"B23","first-page":"454","volume":"39","author":"Ma H","year":"1998","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1042\/bj3070631"},{"key":"B25","first-page":"263","volume":"18","author":"Murachi T.","year":"1989","journal-title":"Biochem Int"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90368-2"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1139\/y86-030"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1999.9565"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)82240-2"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4781(95)00027-E"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.18.10909"},{"key":"B32","doi-asserted-by":"crossref","first-page":"3690","DOI":"10.4049\/jimmunol.158.8.3690","volume":"158","author":"Squier M","year":"1997","journal-title":"J Immunol"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1998.8871"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118398"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)01246-6"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1006\/jsre.1998.5504"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.2001.280.6.C1561","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:18:06Z","timestamp":1718651886000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.2001.280.6.C1561"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,6,1]]},"references-count":36,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2001,6,1]]}},"alternative-id":["10.1152\/ajpcell.2001.280.6.C1561"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.2001.280.6.c1561","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,6,1]]}}}