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When combined with a paracellular sieving model, these data provided quantitative descriptors of the pathway under control conditions and after exposure to TJ modulators. PEG profiles in both cell lines conformed to a biphasic process involving a restrictive pore (radius 4.3\u20134.5 \u00c5) and a nonrestrictive component responsible for permeability of larger molecules. PEG profiling revealed significant differences between the effects of EGTA and sodium caprate (C10). The restrictive component of EGTA-treated cells lost all size discrimination due to an increase in pore radius. Sodium caprate had no effect on pore radius but increased permeability via a different mechanism possibly involving increased numbers of functional pores. PEG profiling provides a useful tool for probing the functional regulation of the paracellular route.<\/jats:p>","DOI":"10.1152\/ajpcell.2001.281.2.c388","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T20:41:57Z","timestamp":1514148117000},"page":"C388-C397","source":"Crossref","is-referenced-by-count":226,"title":["Functional modeling of tight junctions in intestinal cell monolayers using polyethylene glycol oligomers"],"prefix":"10.1152","volume":"281","author":[{"given":"C. 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