{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T16:59:34Z","timestamp":1790182774154,"version":"4.1.0"},"reference-count":30,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2001,8,1]]},"abstract":"<jats:p> Airways of Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-2Cl<jats:sup>\u2212<\/jats:sup>(NKCC1)-deficient mice (\u2212\/\u2212) were studied in Ussing chambers to determine the role of the basolateral NKCC1 in transepithelial anion secretion. The basal short-circuit current ( I <jats:sub>sc<\/jats:sub>) of tracheae and bronchi from adult mice did not differ between NKCC1\u2212\/\u2212 and normal mice, whereas NKCC1\u2212\/\u2212 tracheae from neonatal mice exhibited a significantly reduced basal I <jats:sub>sc<\/jats:sub>. In normal mouse tracheae, sensitivity to the NKCC1 inhibitor bumetanide correlated inversely with the age of the mouse. In contrast, tracheae from NKCC1\u2212\/\u2212 mice at all ages were insensitive to bumetanide. The anion secretory response to forskolin did not differ between normal and NKCC1\u2212\/\u2212 tissues. However, when larger anion secretory responses were induced with UTP, airways from the NKCC1\u2212\/\u2212 mice exhibited an attenuated response. Ion substitution and drug treatment protocols suggested that HCO[Formula: see text]secretion compensated for reduced Cl<jats:sup>\u2212<\/jats:sup> secretion in NKCC1\u2212\/\u2212 airway epithelia. The absence of spontaneous airway disease or pathology in airways from the NKCC1\u2212\/\u2212 mice suggests that the NKCC1 mutant mice are able to compensate adequately for absence of the NKCC1 protein. <\/jats:p>","DOI":"10.1152\/ajpcell.2001.281.2.c615","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T20:41:57Z","timestamp":1514148117000},"page":"C615-C623","source":"Crossref","is-referenced-by-count":50,"title":["Alterations in airway ion transport in NKCC1-deficient mice"],"prefix":"10.1152","volume":"281","author":[{"given":"B. R.","family":"Grubb","sequence":"first","affiliation":[{"name":"Cystic Fibrosis\/Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, North Carolina 27599-7248"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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