{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T05:40:10Z","timestamp":1751175610656,"version":"3.41.0"},"reference-count":33,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2001,9,1]]},"abstract":"<jats:p>We examined the effects of dissolved nitric oxide (NO) gas on cytoplasmic calcium levels ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) in C6 glioma cells under anoxic conditions. The maximum elevation (27 \u00b1 3 nM) of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>was reached at 10 \u03bcM NO. A second application of NO was ineffective if the first was &gt;0.5 \u03bcM. The NO donor diethylamine\/NO mimicked the effects of NO. Acute exposure of the cells to low calcium levels was without effect on the NO-evoked response. Thapsigargin (TG) increased [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>and was less effective if cells were pretreated with NO. Hemoglobin inhibited the effects of NO at a molar ratio of 10:1. 8-Bromo-cGMP was without effect on the NO-evoked response. If cells were pretreated with TG or exposed chronically to nominal amounts of calcium, NO decreased [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>. The results suggest that C6 glioma cells have two receptors for NO. One receptor (NO<jats:sub>A<\/jats:sub>) elevates [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>and resides on the endoplasmic reticulum (ER). The other receptor (NO<jats:sub>B<\/jats:sub>) decreases [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>and resides on the plasmalemma or the ER. The latter receptor dominates when the level of calcium within intracellular stores is diminished.<\/jats:p>","DOI":"10.1152\/ajpcell.2001.281.3.c876","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T20:38:57Z","timestamp":1514147937000},"page":"C876-C885","source":"Crossref","is-referenced-by-count":3,"title":["Regulation of cytoplasmic calcium levels by two nitric oxide receptors"],"prefix":"10.1152","volume":"281","author":[{"given":"Charles L.","family":"Bowman","sequence":"first","affiliation":[{"name":"Buffalo Institute for Medical Research, Veterans Administration Medical Center, Buffalo 14215-1129; and"},{"name":"Department of Physiology and Biophysical Sciences, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lori","family":"Yohe","sequence":"additional","affiliation":[{"name":"Buffalo Institute for Medical Research, Veterans Administration Medical Center, Buffalo 14215-1129; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James W.","family":"Lohr","sequence":"additional","affiliation":[{"name":"Buffalo Institute for Medical Research, Veterans Administration Medical Center, Buffalo 14215-1129; and"},{"name":"Departments of Medicine and Pharmacology, University at Buffalo, Buffalo, New York 14214-3005"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(08)61108-5"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1136(199611)18:3<161::AID-GLIA1>3.0.CO;2-2"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1136(199907)27:1<22::AID-GLIA3>3.0.CO;2-F"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.3.1149"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1021\/bi00151a033"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.25.3.692"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(91)90002-V"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83641-4"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1989.256.6.C1250"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/S0042-6989(99)00007-3"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb16047.x"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90130-6"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1994.sp020247"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(91)90044-T"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1996.76.4.2307"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(99)01073-3"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V57.5.946.946"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.13-08-03333.1993"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199901000-00001"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(81)76111-1"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199901180-00006"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1136(199908)27:2<171::AID-GLIA7>3.0.CO;2-F"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1007\/BF03401690"},{"key":"B30","first-page":"127","volume":"98","author":"Takemura H","year":"1997","journal-title":"Res Commun Mol Pathol Pharmacol"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1998.71041588.x"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.7.2466"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.84.2.201"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1998.78.1.99"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199709)172:3<296::AID-JCP3>3.0.CO;2-J"},{"key":"B36","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0031-6997(25)06656-6","volume":"39","author":"Waldman SA","year":"1987","journal-title":"Pharmacol Rev"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1136(199906)26:4<280::AID-GLIA2>3.0.CO;2-W"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-05-01767.2000"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(08)63212-4"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.2001.281.3.C876","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T05:18:21Z","timestamp":1751174301000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.2001.281.3.C876"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,9,1]]},"references-count":33,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2001,9,1]]}},"alternative-id":["10.1152\/ajpcell.2001.281.3.C876"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.2001.281.3.c876","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"type":"print","value":"0363-6143"},{"type":"electronic","value":"1522-1563"}],"subject":[],"published":{"date-parts":[[2001,9,1]]}}}