{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T16:54:31Z","timestamp":1761238471073},"reference-count":43,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2001,10,1]]},"abstract":"<jats:p> We report for the first time that cultured nonpigmented human ciliary epithelial (NPE) cell layers transport fluid. Cells were grown to confluence on permeable membrane inserts, and fluid transport across the resulting cell layers was determined by volume clamp at 37\u00b0C. These cell layers translocated fluid from the apical to the basal side at a steady rate of 3.6 \u03bcl \u00b7 h<jats:sup>\u22121<\/jats:sup> \u00b7 cm<jats:sup>\u22122<\/jats:sup>( n = 4) for 8 h. This fluid movement was independent of hydrostatic pressure and was completely inhibited by 1 mM ouabain, suggesting it arose from fluid transport. Mercuric chloride, a nonspecific but potent blocker of Hg<jats:sup>2+<\/jats:sup>-sensitive aquaporins, and aquaporin-1 antisense oligonucleotides both partially inhibited fluid transport across the cell layers, which suggests that water channels have a role in NPE cell homeostasis. In addition, these results suggest that of the two ciliary epithelial layers in tandem, the NPE layer by itself can transport fluid. This cultured layer, therefore, constitutes an interesting model that may be useful for physiological and pharmacological characterization of ciliary epithelial fluid secretion. <\/jats:p>","DOI":"10.1152\/ajpcell.2001.281.4.c1139","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T20:40:49Z","timestamp":1514148049000},"page":"C1139-C1145","source":"Crossref","is-referenced-by-count":23,"title":["Fluid transport by human nonpigmented ciliary epithelial layers in culture: a homeostatic role for aquaporin-1"],"prefix":"10.1152","volume":"281","author":[{"given":"Rajkumar V.","family":"Patil","sequence":"first","affiliation":[{"name":"Departments of Ophthalmology and Visual Sciences and"},{"name":"Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110; and Departments of"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiqiang","family":"Han","sequence":"additional","affiliation":[{"name":"Departments of Ophthalmology and Visual Sciences and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maimaiti","family":"Yiming","sequence":"additional","affiliation":[{"name":"Ophthalmology, Columbia University, New York, New York 10032"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junjie","family":"Yang","sequence":"additional","affiliation":[{"name":"Departments of Ophthalmology and Visual Sciences and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pavel","family":"Iserovich","sequence":"additional","affiliation":[{"name":"Ophthalmology, Columbia University, New York, New York 10032"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin B.","family":"Wax","sequence":"additional","affiliation":[{"name":"Departments of Ophthalmology and Visual Sciences and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Fischbarg","sequence":"additional","affiliation":[{"name":"Physiology and Cellular Biophysics and"},{"name":"Ophthalmology, Columbia University, New York, New York 10032"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1993.1156"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(87)91343-X"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.22.8754"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.44.12.739"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.275.6.C1481"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.3109\/02713689008999615"},{"key":"B7","first-page":"2574","volume":"41","author":"Crook RB","year":"2000","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1999.276.3.C548"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(87)90066-6"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1007\/BF01869942"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1038\/266071a0"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1007\/BF00570332"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)39891-6"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.274.5.C1332"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1076\/0271-3683(200003)2031-9FT242"},{"key":"B16","first-page":"42","volume":"85","author":"Helbig H","year":"1988","journal-title":"Fortschr Ophthalmol"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1996.271.3.C703"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4835(66)80009-X"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(14)78372-2"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(84)90096-4"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1089\/jop.1996.12.253"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(97)00011-7"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.274.2.C543"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1992.262.1.C98"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1994.1092"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1177\/37.9.2549122"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.0573"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(79)90137-8"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1996.0196"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9394(60)91624-X"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.50.33123"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1126\/science.256.5055.385"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1177\/026988119701100310"},{"key":"B36","first-page":"131","volume":"89","author":"Sears ML","year":"1991","journal-title":"Trans Am Ophthalmol Soc"},{"key":"B37","first-page":"3867","volume":"35","author":"Stamer W","year":"1994","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1016\/0300-9629(91)90326-8"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1997.5125"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1999.276.6.C1432"},{"key":"B41","first-page":"3057","volume":"35","author":"Wax MB","year":"1994","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"B42","first-page":"1353","volume":"28","author":"Wiederholt M","year":"1987","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1993.1054"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.4.2686"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.84.2.199"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.2001.281.4.C1139","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:14:46Z","timestamp":1718651686000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.2001.281.4.C1139"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,10,1]]},"references-count":43,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2001,10,1]]}},"alternative-id":["10.1152\/ajpcell.2001.281.4.C1139"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.2001.281.4.c1139","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,10,1]]}}}