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These agents promoted apoptosis (i.e., increased expression of annexin V) of wild-type (WT) S49 cells, but Bcl-2-overexpressing S49 cells were protected from this response. Bcl-2 overexpression did not protect cells from G<jats:sub>1<\/jats:sub>growth arrest but did allow cells to grow longer in culture and protected cells from culture-dependent necrosis. Commitment to and reversal from apoptosis vs. G<jats:sub>1<\/jats:sub>growth arrest by isoproterenol + 3-isobutyl-1-methylxanthine showed different kinetics. Although both processes required several hours to develop, removal of agonists readily reversed growth arrest, but not apoptosis. Thus commitment to apoptosis is less reversible than G<jats:sub>1<\/jats:sub>growth arrest. The findings also indicate that glucocorticoid- and cAMP-mediated G<jats:sub>1<\/jats:sub>growth arrest is unaffected by Bcl-2 overexpression, even though increased Bcl-2 allows these lymphoma cells to resist necrosis and apoptosis.<\/jats:p>","DOI":"10.1152\/ajpcell.2001.281.5.c1642","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T20:35:19Z","timestamp":1514147719000},"page":"C1642-C1647","source":"Crossref","is-referenced-by-count":35,"title":["Bcl-2 protects lymphoma cells from apoptosis but not growth arrest promoted by cAMP and dexamethasone"],"prefix":"10.1152","volume":"281","author":[{"given":"Lingzhi","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Pharmacology, University of California, San Diego, La Jolla, California 92093-0636"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul A.","family":"Insel","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of California, San Diego, La Jolla, California 92093-0636"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.18.1.309"},{"key":"B2","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/S0026-895X(25)09315-0","volume":"36","author":"Barber R","year":"1989","journal-title":"Mol Pharmacol"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.8.9.8005391"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1006\/excr.2000.4836"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.15.1899"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1202339"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/0960-0760(94)90272-0"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)43957-3"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.2949"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1042\/bst0280051"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.16.11735"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.86.1.76"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.3922"},{"key":"B14","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/S0026-895X(25)08840-6","volume":"42","author":"Proll MA","year":"1992","journal-title":"Mol Pharmacol"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1210\/endo.141.6.7523"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2000.278.6.H1948"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)32889-2"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2400941"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1096\/fj.00-0327com"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)01761-5"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(87)51005-9"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1038\/70237"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2000.279.5.C1665"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.2001.281.5.C1642","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T05:18:10Z","timestamp":1751174290000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.2001.281.5.C1642"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,11,1]]},"references-count":23,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2001,11,1]]}},"alternative-id":["10.1152\/ajpcell.2001.281.5.C1642"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.2001.281.5.c1642","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,11,1]]}}}