{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T00:43:12Z","timestamp":1771634592611,"version":"3.50.1"},"reference-count":30,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2002,1,1]]},"abstract":"<jats:p>Micromolar concentrations of ATP stimulate biphasic change in transepithelial conductance across CaSki cultures, an acute increase (phase I response) followed by a slower decrease ( phase II response). Phase I and phase II responses involve two distinct calcium-dependent pathways, calcium mobilization and calcium influx. To test the hypothesis that phase I and phase II responses are mediated by distinct P2 purinergic receptors, changes in permeability were uncoupled by blocking calcium mobilization with 1,2-bis(2-aminophenoxy)ethane- N,N,N\u2032,N\u2032-tetraacetic acid (BAPTA) or by lowering extracellular calcium, respectively. Under these conditions ATP EC<jats:sub>50<\/jats:sub>was 25 \u03bcM for phase Iresponse and 2 \u03bcM for phase II response. The respective agonist profiles were ATP &gt; UTP &gt; adenosine 5\u2032- O-(3-thiotriphosphate) (ATP-\u03b3S) = N<jats:sup>6<\/jats:sup>-([6-aminohexyl]carbamoylmethyl)adenosine 5\u2032-triphosphate (A8889) &gt; GTP and UTP &gt; ATP &gt; GTP = A8889 &gt; ATP-\u03b3S. Suramin blocked phase Iresponse and ATP-induced calcium mobilization, whereas pyridoxal phosphate-6-azophenyl-2\u2032,4-disulfonic acid (PPADS) blocked phase II response and ATP-augmented calcium influx. ATP time course and pharmacological profiles for phase II response and augmented calcium influx were similar, with a time constant of 2 min and a saturable concentration-dependent effect (EC<jats:sub>50<\/jats:sub>of 2\u20133 \u03bcM). RT-PCR experiments revealed expression of mRNA for both the P2Y<jats:sub>2<\/jats:sub>and P2X<jats:sub>4<\/jats:sub>receptors. These results suggest that the ATP-induced phase I and phase IIresponses are mediated by distinct P2 purinergic receptor mechanisms.<\/jats:p>","DOI":"10.1152\/ajpcell.2002.282.1.c75","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T20:28:11Z","timestamp":1514147291000},"page":"C75-C83","source":"Crossref","is-referenced-by-count":21,"title":["Regulation of transcervical permeability by two distinct P2 purinergic receptor mechanisms"],"prefix":"10.1152","volume":"282","author":[{"given":"George I.","family":"Gorodeski","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, University MacDonald Women's Hospital, University Hospitals of Cleveland, and Departments of Reproductive Biology and Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703848"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1007\/s004410050010"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb15457.x"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a019021"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1929.sp002608"},{"key":"B6","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/S0026-895X(25)10232-0","volume":"48","author":"Evans RJ","year":"1995","journal-title":"Mol Pharmacol"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1124\/mol.51.1.109"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.1998.tb02257.x"},{"key":"B10","first-page":"2584","volume":"85","author":"Gorodeski GI.","year":"2000","journal-title":"J Clin Endocrinol Metab"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.275.3.C758"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.272.5.C1602"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(97)00471-8"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.268.5.C1215"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1007\/BF02737899"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1177\/107155769400100309"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1996.270.6.C1715"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-0436.1994.56120107.x"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.272.2.C511"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1007\/BF02737900"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pa.35.040195.002545"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1990.tb12979.x"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1983.sp014865"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.40.1.563"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.8.3275"},{"key":"B26","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/S0031-6997(24)01373-5","volume":"50","author":"Ralevic V","year":"1998","journal-title":"Pharmacol Rev"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(84)81152-7"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1023\/A:1004017714702"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1080001"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1951.tb00800.x"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(95)02252-X"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.2002.282.1.C75","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T05:17:49Z","timestamp":1751174269000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.2002.282.1.C75"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,1,1]]},"references-count":30,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2002,1,1]]}},"alternative-id":["10.1152\/ajpcell.2002.282.1.C75"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.2002.282.1.c75","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,1,1]]}}}