{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:12:56Z","timestamp":1760188376609},"reference-count":44,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:p>We examined whether plasma concentrations of nonglucose insulin secretagogues are associated with prehepatic insulin secretion rates (ISR) in nondiabetic, insulin-resistant, human immunodeficiency virus (HIV)-infected, lipodystrophic patients (LIPO). Additionally, the negative feedback of insulin on ISR was evaluated. ISR were estimated by deconvolution of plasma C-peptide concentrations during fasting (basal) and during the last 30 min of a 120-min euglycemic insulin clamp (40 mU\u00b7m<jats:sup>\u22122<\/jats:sup>\u00b7min<jats:sup>\u22121<\/jats:sup>). Eighteen normoglycemic LIPO were compared with 25 normoglycemic HIV-infected patients without lipodystrophy (controls). Thirty minutes before start of the clamp, a bolus of glucose was injected intravenously to stimulate endogenous insulin secretion. Insulin sensitivity index (Si<jats:sub>R<jats:sub>d<\/jats:sub><\/jats:sub>) was estimated from glucose tracer analysis. LIPO displayed increased basal ISR (69%), clamp ISR (114%), basal insulin (130%), and clamp insulin (32%), all P \u2264 0.001, whereas Si<jats:sub>R<jats:sub>d<\/jats:sub><\/jats:sub>was decreased (57%, P &lt; 0.001). In LIPO, ISR<jats:sub>basal<\/jats:sub>correlated significantly with basal insulin, alanine, and glucagon (all r &gt; 0.65, P &lt; 0.01), but not with glucose. In control subjects, ISR<jats:sub>basal<\/jats:sub>correlated significantly with insulin, glucagon, and glucose (all r &gt; 0.41, P &lt; 0.05), but not with alanine. In LIPO, ISR<jats:sub>clamp<\/jats:sub>correlated significantly with clamp free fatty acids (FFA), alanine, triglyceride, and glucagon (all r &gt; 0.51, P &lt; 0.05). In control subjects, ISR<jats:sub>clamp<\/jats:sub>correlated with clamp triglyceride ( r = 0.45, P &lt; 0.05). Paradoxically, in LIPO, ISR<jats:sub>clamp<\/jats:sub>correlated positively with clamp insulin ( r = 0.68, P &lt; 0.01), which suggests an absent negative feedback of insulin on ISR. Our data support evidence that lipodystrophic, nondiabetic, HIV-infected patients exhibit increased ISR, which can be partially explained by an impaired negative feedback of insulin on \u03b2-cells and an increased stimulation of ISR by FFA, alanine, triglyceride, and glucagon.<\/jats:p>","DOI":"10.1152\/ajpendo.00009.2004","type":"journal-article","created":{"date-parts":[[2004,5,25]],"date-time":"2004-05-25T00:23:23Z","timestamp":1085444603000},"page":"E677-E685","source":"Crossref","is-referenced-by-count":21,"title":["Insulin secretion in lipodystrophic HIV-infected patients is associated with high levels of nonglucose secretagogues and insulin resistance of \u03b2-cells"],"prefix":"10.1152","volume":"287","author":[{"given":"Steen 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