{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:29:13Z","timestamp":1761488953971},"reference-count":36,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[2012,9,1]]},"abstract":"<jats:p> The prevalence of obesity is increasing globally, and obesity is a major risk factor for metabolic diseases such as type 2 diabetes. Previously, we reported that oral administration of homobrassinolide (HB) to healthy rats triggered a selective anabolic response that was associated with lower blood glucose. Therefore, the aim of this study was to evaluate the effects of HB administration on glucose metabolism, insulin sensitivity, body composition, and gluconeogenic gene expression profiles in liver of C57BL\/6J high-fat diet-induced obese mice. Acute oral administration of 50\u2013300 mg\/kg HB to obese mice resulted in a dose-dependent decrease in fasting blood glucose within 3 h of treatment. Daily chronic administration of HB (50 mg\/kg for 8 wk) ameliorated hyperglycemia and improved oral glucose tolerance associated with obesity without significantly affecting body weight or body composition. These changes were accompanied by lower expression of two key gluconeogenic enzymes, phospho enolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G-6-Pase), and increased phosphorylation of AMP-activated protein kinase in the liver and muscle tissue. In vitro, HB treatment (1\u201315 \u03bcM) inhibited cyclic AMP-stimulated but not dexamethasone-stimulated upregulation of PEPCK and G-6-Pase mRNA levels in H4IIE rat hepatoma cells. Among a series of brassinosteroid analogs related to HB, only homocastasterone decreased glucose production in cell culture significantly. These results indicate the antidiabetic effects of brassinosteroids and begin to elucidate their putative cellular targets both in vitro and in vivo. <\/jats:p>","DOI":"10.1152\/ajpendo.00024.2012","type":"journal-article","created":{"date-parts":[[2012,7,12]],"date-time":"2012-07-12T02:54:33Z","timestamp":1342061673000},"page":"E652-E658","source":"Crossref","is-referenced-by-count":13,"title":["Hypoglycemic effects of brassinosteroid in diet-induced obese mice"],"prefix":"10.1152","volume":"303","author":[{"given":"Debora","family":"Esposito","sequence":"first","affiliation":[{"name":"Biotech Center, School of Environmental and Biological Sciences, Rutgers University, New Brunswick, New Jersey; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pablo","family":"Kizelsztein","sequence":"additional","affiliation":[{"name":"Biotech Center, School of Environmental and Biological Sciences, Rutgers University, New Brunswick, New Jersey; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Slavko","family":"Komarnytsky","sequence":"additional","affiliation":[{"name":"Biotech Center, School of Environmental and Biological Sciences, Rutgers University, New Brunswick, New Jersey; and"},{"name":"Plants for Human Health Institute, Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, Kannapolis, North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilya","family":"Raskin","sequence":"additional","affiliation":[{"name":"Biotech Center, School of Environmental and Biological Sciences, Rutgers University, New Brunswick, New Jersey; and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.45.11.1563"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(02)00656-8"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(98)00367-7"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.2174\/092986708783330674"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M602416200"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.90596.2008"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1007\/s003359901063"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.47.6.882"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/S0083-6729(02)65065-4"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ijo.0802928"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1096\/fj.11-181271"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1021\/jm200028h"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.54.031902.134921"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1021\/jf073059z"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00420.2007"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1038\/281216a0"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.66.1.581"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1970"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.2337\/db07-0381"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-137-1-200207020-00009"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.90772.2008"},{"key":"B22","unstructured":"Kuzmitsky BB , Mizulo NA . 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