{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T21:37:42Z","timestamp":1693345062243},"reference-count":64,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[2013,7,1]]},"abstract":"<jats:p> In the present work, we investigated the effect of 17\u03b2-estradiol (E<jats:sub>2<\/jats:sub>) and basic fibroblast growth factor 2 (FGF2) on the lactotroph cell-proliferative response and the related membrane-initiated signaling pathway. Anterior pituitary mixed-cell cultures of random, cycling 3-mo-old female rats were treated with 10 nM E<jats:sub>2<\/jats:sub>, E<jats:sub>2<\/jats:sub> membrane-impermeable conjugated BSA (E<jats:sub>2<\/jats:sub>-BSA), PPT (ER\u03b1 agonist), and DPN (ER\u03b2 agonist) alone or combined with FGF2 (10 ng\/ml) for 30 min or 4 h. Although our results showed that the uptake of BrdU into the nucleus of lactotrophs was not modified by E<jats:sub>2<\/jats:sub> or FGF2 alone, a significant increase in the lactotroph uptake of BrdU was observed after E<jats:sub>2<\/jats:sub>\/FGF2 coincubation, with this effect being mimicked by PPT\/FGF2. These proliferative effects were blocked by ICI 182,780 or PD-98059. The involvement of membrane ER in the proliferative response of prolactin cells induced by the steroid and FGF2 coincubation was confirmed using E<jats:sub>2<\/jats:sub>-BSA, and the association between ER\u03b1 and FGF receptor was observed after E<jats:sub>2<\/jats:sub>\/FGF2 treatment by immunoprecipitation. A significant increase in the ERK1\/2 expression was noted after E<jats:sub>2<\/jats:sub>, E<jats:sub>2<\/jats:sub>-BSA, PPT, and FGF2 alone, which was more noticeable after E<jats:sub>2<\/jats:sub>-BSA\/FGF2, E<jats:sub>2<\/jats:sub>\/FGF2, or PPT\/FGF2 treatments. This study provides evidence that E<jats:sub>2<\/jats:sub> and FGF2 exert a cooperative effect on the lactotroph proliferation principally by signaling initiated at the plasma membrane triggering a genomic effect mediated by MEK\/ERK1\/2, a common signaling pathway, that finally regulates the lactotroph population, thus contributing to pituitary plasticity. <\/jats:p>","DOI":"10.1152\/ajpendo.00027.2013","type":"journal-article","created":{"date-parts":[[2013,5,8]],"date-time":"2013-05-08T01:43:50Z","timestamp":1367977430000},"page":"E41-E49","source":"Crossref","is-referenced-by-count":7,"title":["Cooperative effect of E<sub>2<\/sub> and FGF2 on lactotroph proliferation triggered by signaling initiated at the plasma membrane"],"prefix":"10.1152","volume":"305","author":[{"given":"Liliana del V.","family":"Sosa","sequence":"first","affiliation":[{"name":"Centro de Microscop\u00eda Electr\u00f3nica, Instituto de Investigaciones en Ciencias de la Salud-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Facultad de Ciencias M\u00e9dicas, Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvina","family":"Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Centro de Microscop\u00eda Electr\u00f3nica, Instituto de Investigaciones en Ciencias de la Salud-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Facultad de Ciencias M\u00e9dicas, Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan P.","family":"Petiti","sequence":"additional","affiliation":[{"name":"Centro de Microscop\u00eda Electr\u00f3nica, Instituto de Investigaciones en Ciencias de la Salud-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Facultad de Ciencias M\u00e9dicas, Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alicia M.","family":"Vaca","sequence":"additional","affiliation":[{"name":"Centro de Microscop\u00eda Electr\u00f3nica, Instituto de Investigaciones en Ciencias de la Salud-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Facultad de Ciencias M\u00e9dicas, Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana L.","family":"De Paul","sequence":"additional","affiliation":[{"name":"Centro de Microscop\u00eda Electr\u00f3nica, Instituto de Investigaciones en Ciencias de la Salud-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Facultad de Ciencias M\u00e9dicas, Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alicia I.","family":"Torres","sequence":"additional","affiliation":[{"name":"Centro de Microscop\u00eda Electr\u00f3nica, Instituto de Investigaciones en Ciencias de la Salud-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Facultad de Ciencias M\u00e9dicas, Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.82.4.3896"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2826.2005.01283.x"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1210\/edrv.22.6.0451"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4547(20000101)59:1<107::AID-JNR13>3.0.CO;2-W"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.17.5364"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.7175"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00349.2004"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00260.2007"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(00)01676-X"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1210\/en.2004-1448"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.105.088302"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00133.2009"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2826.2007.01567.x"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2001.012947"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.5.2325"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1023\/A:1026322130243"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2010.054502"},{"key":"B18","first-page":"65","volume-title":"Biochemistry, Genetics and Molecular Biology,","author":"De Paul AL","year":"2012"},{"key":"B19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1365-2826.2008.01676.x","volume":"20","author":"Denef C","year":"2008","journal-title":"J Neuroendocrinol"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.16.5773"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2005.08.005"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2008.10.014"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1210\/me.2007-0419"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2008.05.001"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01732-06"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2008.01.002"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-006-0283-4"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-005-0038-4"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2184.2010.00700.x"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2012.02.008"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1110367"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2005.09.006"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1210\/endo.141.3.7382"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1076\/apab.110.1.50.911"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1210\/en.2006-1484"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1210\/en.2006-1329"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109592200"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1990.70.2.279"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-11-2547"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2008.12.014"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(03)00088-3"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1007\/0-387-26274-1_12"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1016\/j.yfrne.2006.09.001"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1016\/j.yfrne.2012.07.003"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1210\/endo.139.9.6181"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041310118"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-004-0661-5"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-006-0101-9"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1570053"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1340241"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2008.04.015"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1007\/s10735-010-9255-9"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1159\/000111559"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(02)03186-4"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1208076"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1210\/me.2007-0109"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0308334100"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00408.2011"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1210\/endo.138.5.5142"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2009.09.006"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2005.02.007"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1159\/000054574"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0041299"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.90785.2008"}],"container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpendo.00027.2013","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T16:05:34Z","timestamp":1567958734000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpendo.00027.2013"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,1]]},"references-count":64,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,7,1]]}},"alternative-id":["10.1152\/ajpendo.00027.2013"],"URL":"https:\/\/doi.org\/10.1152\/ajpendo.00027.2013","relation":{},"ISSN":["0193-1849","1522-1555"],"issn-type":[{"value":"0193-1849","type":"print"},{"value":"1522-1555","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,1]]}}}