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To accomplish this, we generated transgenic mice harboring a luciferase reporter gene under the control of IL-6 gene regulatory regions to allow accurate quantification of IL-6 gene activity in vivo. In these mice, induction of secondary hyperparathyroidism using a calcium-deficient diet did not alter IL-6-luciferase transgene expression, whereas RANKL mRNA expression was elevated in bone tissue. Moreover, secondary hyperparathyroidism induced an equivalent amount of bone loss in wild-type and IL-6-deficient mice, and PTH elevated RANKL mRNA and osteoclast formation to the same extent in bone marrow cultures derived from wild-type and IL-6-deficient mice. These results demonstrate that IL-6 is not required for the osteoclast formation and bone loss that accompanies continuous elevation of PTH.<\/jats:p>","DOI":"10.1152\/ajpendo.00029.2005","type":"journal-article","created":{"date-parts":[[2005,6,15]],"date-time":"2005-06-15T03:53:18Z","timestamp":1118807598000},"page":"E784-E793","source":"Crossref","is-referenced-by-count":58,"title":["IL-6 is not required for parathyroid hormone stimulation of RANKL expression, osteoclast formation, and bone loss in mice"],"prefix":"10.1152","volume":"289","author":[{"given":"Charles A.","family":"O'Brien","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert L.","family":"Jilka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott","family":"Stewart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert S.","family":"Weinstein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stavros C.","family":"Manolagas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.10.3.1192"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116872"},{"key":"R3","unstructured":"Barnicot NA.The local action of the parathyroid and other tissues on bone in intracerebral grafts.J Anat82: 233\u2013248, 1948."},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1007\/BF02008077"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M307444200"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198002213020801"},{"key":"R7","unstructured":"Burr DB.Bone quality: understanding what matters (Review; 28 refs).J Musculoskelet Neuronal Interact4: 184\u2013186, 2004."},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-197759070-00021"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117990"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1998.13.3.393"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1210\/endo.138.11.5505"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00180.2004"},{"key":"R13","doi-asserted-by":"crossref","unstructured":"Fraher LJ, Avram R, Watson PH, Hendy GN, Henderson JE, Chong KL, Goltzman D, Morley P, Willick GE, Whitfield JF, and Hodsman AB.Comparison of the biochemical responses to human parathyroid hormone-(1\u201331)NH2 and hPTH-(1\u201334) in healthy humans.J Clin Endocrinol Metab84: 2739\u20132743, 1999.","DOI":"10.1210\/jcem.84.8.5891"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M208494200"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1096\/fj.99-1075com"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650081003"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1210\/endo.140.10.7036"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.232562799"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1210\/endo-101-3-969"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2000.15.9.1766"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1997.12.5.820"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1126\/science.1621100"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118600"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1210\/endo.142.12.8536"},{"key":"R25","unstructured":"Kitamura H, Kawata H, Takahashi F, Higuchi Y, Furuichi T, and Ohkawa H.Bone marrow neutrophilia and suppressed bone turnover in human interleukin-6 transgenic mice. 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