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In the present investigation we evaluated whether impaired mitochondrial phosphorylation and inflammation also occur in endocrine pancreas of anorectic mice, and if glucose homeostasis is disturbed. We show that anx\/ anx mice exhibit marked glucose intolerance associated with reduced insulin release following an intraperitoneal injection of glucose. In contrast, insulin release from isolated anx\/ anx islets is increased after stimulation with glucose or KCl. In isolated anx\/ anx islets there is a strong downregulation of the mitochondrial complex I (CI) assembly factor, NADH dehydrogenase (ubiquinone) 1\u03b1 subcomplex, assembly factor 1 ( Ndufaf1), and a reduced CI activity. In addition, we show elevated concentrations of free fatty acids (FFAs) in anx\/ anx serum and increased macrophage infiltration (indicative of inflammation) in anx\/ anx islets. However, isolated islets from anx\/ anx mice cultured in the absence of FFAs do not exhibit increased inflammation. We conclude that the phenotype of the endocrine pancreas of the anx\/ anx mouse is characterized by increased levels of circulating FFAs, as well as inflammation, which can inhibit insulin secretion in vivo. The anx\/ anx mouse may represent a useful tool for studying molecular mechanisms underlying the association between diabetes and eating disorders.<\/jats:p>","DOI":"10.1152\/ajpendo.00081.2015","type":"journal-article","created":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T06:15:30Z","timestamp":1435731330000},"page":"E418-E427","source":"Crossref","is-referenced-by-count":14,"title":["Glucose intolerance and pancreatic \u03b2-cell dysfunction in the anorectic<i>anx<\/i>\/<i>anx<\/i>mouse"],"prefix":"10.1152","volume":"309","author":[{"given":"Charlotte","family":"Lindfors","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden;"},{"name":"Center for Molecular Medicine, Karolinska University Hospital, Stockholm, Sweden;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abram","family":"Katz","sequence":"additional","affiliation":[{"name":"Department of Physiology &amp; Pharmacology, Karolinska Institutet, Stockholm, Sweden;"},{"name":"Ariel University, Department of Physical Therapy, Ariel, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Selander","sequence":"additional","affiliation":[{"name":"The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeanette E.","family":"Johansen","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden;"},{"name":"Center for Molecular Medicine, Karolinska University Hospital, Stockholm, Sweden;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Marconi","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden;"},{"name":"Center for Molecular Medicine, Karolinska University Hospital, Stockholm, Sweden;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Schalling","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden;"},{"name":"Center for Molecular Medicine, Karolinska University Hospital, Stockholm, Sweden;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomas","family":"H\u00f6kfelt","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Per-Olof","family":"Berggren","sequence":"additional","affiliation":[{"name":"The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergei","family":"Zaitsev","sequence":"additional","affiliation":[{"name":"The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden;"},{"name":"Belozersky Institute of Physico-Chemical Biology and Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ida A. 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