{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T03:35:31Z","timestamp":1792812931745,"version":"4.5.0"},"reference-count":50,"publisher":"American Physiological Society","issue":"2","funder":[{"DOI":"10.13039\/100000041","name":"American Diabetes Association (ADA)","doi-asserted-by":"publisher","award":["ADA 7-04-JF-36"],"award-info":[{"award-number":["ADA 7-04-JF-36"]}],"id":[{"id":"10.13039\/100000041","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008745","name":"Alabama Agricultural Experiment Station","doi-asserted-by":"crossref","award":["ALA080-052"],"award-info":[{"award-number":["ALA080-052"]}],"id":[{"id":"10.13039\/100008745","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Malone Zallen Graduate Reseaerch Fellowship","award":["MZRF10-01"],"award-info":[{"award-number":["MZRF10-01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[2019,8,1]]},"abstract":"<jats:p>Fetuin-A (Fet-A), a hepatokine associated with insulin resistance, obesity, and incident type 2 diabetes, is shown to exist in both phosphorylated and dephosphorylated forms in circulation. However, studies on fetuin-A phosphorylation status in insulin-resistant conditions and its functional significance are limited. We demonstrate that serum phosphofetuin-A (Ser312) levels were significantly elevated in high-fat diet-induced obese mice, insulin-resistant Zucker diabetic fatty rats, and in individuals with obesity who are insulin resistant. Unlike serum total fetuin-A, serum phosphofetuin-A was associated with body weight, insulin, and markers of insulin resistance. To characterize potential mechanisms, fetuin-A was purified from Hep3B human hepatoma cells. Hep3B Fet-A was phosphorylated (Ser312) and inhibited insulin-stimulated glucose uptake and glycogen synthesis in L6GLUT4 myoblasts. Furthermore, single (Ser312Ala) and double (Ser312Ala + Ser120Ala) phosphorylation-defective Fet-A mutants were without effect on glucose uptake and glycogen synthesis in L6GLUT4 myoblasts. Together, our studies demonstrate that phosphorylation status of Fet-A (Ser312) is associated with obesity and insulin resistance and raise the possibility that Fet-A phosphorylation may play a role in regulation of insulin action.<\/jats:p>","DOI":"10.1152\/ajpendo.00089.2018","type":"journal-article","created":{"date-parts":[[2019,5,14]],"date-time":"2019-05-14T20:46:28Z","timestamp":1557866788000},"page":"E250-E260","source":"Crossref","is-referenced-by-count":29,"title":["Phosphorylation status of fetuin-A is critical for inhibition of insulin action and is correlated with obesity and insulin resistance"],"prefix":"10.1152","volume":"317","author":[{"given":"Guang","family":"Ren","sequence":"first","affiliation":[{"name":"Department of Nutrition and Dietetics, Auburn University, Auburn, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teayoun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Dietetics, Auburn University, Auburn, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James B.","family":"Papizan","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Dietetics, Auburn University, Auburn, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl K.","family":"Okerberg","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Dietetics, Auburn University, Auburn, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vishal M.","family":"Kothari","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Dietetics, Auburn University, Auburn, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilal","family":"Zaid","sequence":"additional","affiliation":[{"name":"Cell Biology Program, The Hospital for Sick Children, Toronto, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillip J.","family":"Bilan","sequence":"additional","affiliation":[{"name":"Cell Biology Program, The Hospital for Sick Children, Toronto, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Araya-Ramirez","sequence":"additional","affiliation":[{"name":"School of Kinesiology, Auburn University, Auburn, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurel A.","family":"Littlefield","sequence":"additional","affiliation":[{"name":"School of Kinesiology, Auburn University, Auburn, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert L.","family":"Bowers","sequence":"additional","affiliation":[{"name":"School of Kinesiology, Auburn University, Auburn, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. Jack","family":"Mahurin","sequence":"additional","affiliation":[{"name":"School of Kinesiology, Auburn University, Auburn, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary M.","family":"Nickles","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Dietetics, Samford University, Birmingham, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca","family":"Ludvigsen","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Dietetics, Auburn University, Auburn, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoming","family":"He","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Dietetics, Auburn University, Auburn, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter W.","family":"Grandjean","sequence":"additional","affiliation":[{"name":"School of Kinesiology, Auburn University, Auburn, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suresh T.","family":"Mathews","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Dietetics, Auburn University, Auburn, Alabama"},{"name":"Department of Nutrition and Dietetics, Samford University, Birmingham, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1007\/s00592-008-0033-z"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2016.10.002"},{"key":"B3","doi-asserted-by":"crossref","first-page":"15925","DOI":"10.1016\/S0021-9258(17)40769-1","volume":"269","author":"Akhoundi C","year":"1994","journal-title":"J Biol Chem"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.2337\/db07-0558"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(89)90293-8"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90098-6"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1042\/bj3640173"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-377-1:155"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2010-0148"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C113.495473"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1210\/endo.139.10.6258"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20100330"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2012.12.011"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-031210-101322"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.4324\/9780203302958_chapter_15"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.105.169404"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1042\/0264-6021:3570437"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1530\/EJE-11-0864"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2869"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.51.7.2090"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.5551\/jat.3830"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.105.588723"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1001\/jama.300.2.182"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1994.tb20026.x"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.01.041"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.85.7.6661"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1002\/dmrr.967"},{"key":"B28","doi-asserted-by":"crossref","first-page":"15965","DOI":"10.1016\/S0021-9258(17)36353-6","volume":"260","author":"Le Cam A","year":"1985","journal-title":"J Biol Chem"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1172\/JCI57132"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(00)00237-9"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.09.071"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.51.8.2450"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1007\/BF00280883"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.49.31735"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1042\/bj3500589"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1038\/nri2921"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2851"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2012.49"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-016-1132-1"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.54.8.2477"},{"key":"B41","doi-asserted-by":"crossref","first-page":"5704","DOI":"10.1016\/S0021-9258(20)79875-3","volume":"249","author":"Spiro RG","year":"1974","journal-title":"J Biol Chem"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1016\/S0898-6568(96)00110-6"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.1417"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1210\/mend.7.11.7906861"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.2337\/db08-0538"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3116"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2012.258"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2008.614"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(95)00063-Z"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.2337\/db17-1348"}],"container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpendo.00089.2018","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,18]],"date-time":"2022-09-18T01:07:06Z","timestamp":1663463226000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpendo.00089.2018"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,1]]},"references-count":50,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,8,1]]}},"alternative-id":["10.1152\/ajpendo.00089.2018"],"URL":"https:\/\/doi.org\/10.1152\/ajpendo.00089.2018","relation":{},"ISSN":["0193-1849","1522-1555"],"issn-type":[{"value":"0193-1849","type":"print"},{"value":"1522-1555","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,1]]}}}