{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T14:06:29Z","timestamp":1648994789852},"reference-count":35,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[2012,7,15]]},"abstract":"<jats:p> Expression of GFP in GnRH neurons has allowed for studies of individual GnRH neurons. We have demonstrated previously the preservation of physiological function in male GnRH-GFP mice. In the present study, we confirm using biocytin-filled GFP-positive neurons in the hypothalamic slice preparation that GFP-expressing somata, axons, and dendrites in hypothalamic slices from GnRH-GFP rats are GnRH1 peptide positive. Second, we used repetitive sampling to study hormone secretion from GnRH-GFP transgenic rats in the homozygous, heterozygous, and wild-type state and between transgenic and Wistar males after \u223c4 yr of backcrossing. Parameters of hormone secretion were not different between the three genetic groups or between transgenic males and Wistar controls. Finally, we performed long-term recording in as many GFP-identified GnRH neurons as possible in hypothalamic slices to determine their patterns of discharge. In some cases, we obtained GnRH neuronal recordings from individual males in which blood samples had been collected the previous day. Activity in individual GnRH neurons was expressed as total quiescence, a continuous pattern of firing of either low or relatively high frequencies or an intermittent pattern of firing. In males with both intensive blood sampling (at 6-min intervals) and recordings from their GnRH neurons, we analyzed the activity of GnRH neurons with intermittent activity above 2 Hz using cluster analysis on both data sets. The average number of pulses was 3.9 \u00b1 0.6\/h. The average number of episodes of firing was 4.0 \u00b1 0.6\/h. Therefore, the GnRH pulse generator may be maintained in the sagittal hypothalamic slice preparation. <\/jats:p>","DOI":"10.1152\/ajpendo.00157.2012","type":"journal-article","created":{"date-parts":[[2012,5,24]],"date-time":"2012-05-24T00:59:47Z","timestamp":1337821187000},"page":"E243-E252","source":"Crossref","is-referenced-by-count":6,"title":["Hormone secretion in transgenic rats and electrophysiological activity in their gonadotropin releasing-hormone neurons"],"prefix":"10.1152","volume":"303","author":[{"given":"Vernon L.","family":"Gay","sequence":"first","affiliation":[{"name":"Department of Cellular and Molecular Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Hemond","sequence":"additional","affiliation":[{"name":"University of Texas San Antonio Department of Biology, San Antonio, Texas;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deena","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Ohio State University, Columbus, Ohio;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael P.","family":"O'Boyle","sequence":"additional","affiliation":[{"name":"University of Texas San Antonio Department of Biology, San Antonio, Texas;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zoe","family":"Hemond","sequence":"additional","affiliation":[{"name":"University of Texas San Antonio Department of Biology, San Antonio, Texas;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janet","family":"Best","sequence":"additional","affiliation":[{"name":"Ohio State University, Columbus, Ohio;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"O'Farrell","sequence":"additional","affiliation":[{"name":"Georgia Institute of Technology, Atlanta, Georgia; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelly J.","family":"Suter","sequence":"additional","affiliation":[{"name":"University of Texas San Antonio Department of Biology, San Antonio, Texas;"},{"name":"University of Texas San Antonio Neurosciences Institute, San Antonio, Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.20.9630"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4738-06.2007"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1126\/science.2459774"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1210\/endo-110-5-1618"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod30.3.619"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1984.247.1.E130"},{"key":"B7","volume-title":"The Rat Brain in Stereotaxic Coordinates","author":"Franklin KBJ","year":"1998"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1210\/endo-90-1-158"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1210\/endo-128-2-965"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(99)01820-X"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1530\/eje.0.1330585"},{"key":"B12","first-page":"36","volume":"23","author":"Hemond PJ","year":"2010","journal-title":"J Vis Exp"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-1139"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(92)90324-3"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1210\/en.2003-0213"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/0361-9230(84)90112-6"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1159\/000124988"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/0301-0082(92)90032-A"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1210\/endo-122-5-2211"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1982.243.4.E310"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1210\/endo.143.6.8869"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1210\/endo-129-3-1207"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1210\/endo.133.4.8404590"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.13.5813"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1998.274.4.E665"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0501832102"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1159\/000124641"},{"key":"B29","first-page":"1683","volume-title":"Physiology of Reproduction","author":"Silverman AJ","year":"1994"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-06-02037.1999"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-24-08578.2003"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1210\/en.2003-1374"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.90300.2008"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1210\/endo.141.1.7279"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1986.250.4.E486"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2826.2008.01697.x"}],"container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpendo.00157.2012","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T19:55:18Z","timestamp":1567972518000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpendo.00157.2012"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,7,15]]},"references-count":35,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,7,15]]}},"alternative-id":["10.1152\/ajpendo.00157.2012"],"URL":"https:\/\/doi.org\/10.1152\/ajpendo.00157.2012","relation":{},"ISSN":["0193-1849","1522-1555"],"issn-type":[{"value":"0193-1849","type":"print"},{"value":"1522-1555","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,7,15]]}}}