{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T10:37:28Z","timestamp":1778841448247,"version":"3.51.4"},"reference-count":62,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[2013,8,1]]},"abstract":"<jats:p>Carnitine palmitoyltransferase-1 (CPT-1) liver isoform, or CPT-1a, is implicated in CNS control of food intake. However, the exact brain nucleus site(s) in mediating this action of CPT-1a has not been identified. In this report, we assess the role of CPT-1a in hypothalamic ventromedial nucleus (VMN). We stereotaxically injected an adenoviral vector containing CPT-1a coding sequence into the VMN of rats to induce overexpression and activation of CPT-1a. The VMN-selective activation of CPT-1a induced an orexigenic effect, suggesting CPT-1a in the VMN is involved in the central control of feeding. Intracerebroventricular administration of etomoxir, a CPT-1 inhibitor, decreases food intake. Importantly, in the animals with VMN overexpression of a CPT-1a mutant that antagonizes the CPT-1 inhibition by etomoxir, the anorectic response to etomoxir was attenuated. This suggests that VMN is involved in mediating the anorectic effect of central inhibition of CPT-1a. In contrast, arcuate nucleus (Arc) overexpression of the mutant did not alter etomoxir-induced inhibition of food intake, suggesting that Arc CPT-1a does not play significant roles in this anorectic action. Furthermore, in the VMN, CPT-1a appears to act downstream of hypothalamic malonyl-CoA action of feeding. Finally, we show that in the VMN CPT-1 activity was altered in concert with fasting and refeeding states, supporting a physiological role of CPT-1a in mediating the control of feeding. All together, CPT-1a in the hypothalamic VMN appears to play an important role in central control of food intake. VMN-selective modulation of CPT-1a activity may therefore be a promising strategy in controlling food intake and maintaining normal body weight.<\/jats:p>","DOI":"10.1152\/ajpendo.00168.2013","type":"journal-article","created":{"date-parts":[[2013,6,5]],"date-time":"2013-06-05T05:57:39Z","timestamp":1370411859000},"page":"E336-E347","source":"Crossref","is-referenced-by-count":11,"title":["Important role of ventromedial hypothalamic carnitine palmitoyltransferase-1a in the control of food intake"],"prefix":"10.1152","volume":"305","author":[{"given":"Su","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Mazankowski Alberta Heart Institute, University of Alberta, Edmonton, Alberta, Canada;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dolors","family":"Serra","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Facultat de Farm\u00e0cia, Universitat de Barcelona, Barcelona, Spain; and"},{"name":"Institut de Biomedicina de la Universitat de Barcelona and CIBER Fisiopatolog\u00eda de la Obesidad y la Nutrici\u00f3n, Instituto de Salud Carlos III, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wendy","family":"Keung","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Mazankowski Alberta Heart Institute, University of Alberta, Edmonton, Alberta, Canada;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fausto G.","family":"Hegardt","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Facultat de Farm\u00e0cia, Universitat de Barcelona, Barcelona, Spain; and"},{"name":"Institut de Biomedicina de la Universitat de Barcelona and CIBER Fisiopatolog\u00eda de la Obesidad y la Nutrici\u00f3n, Instituto de Salud Carlos III, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary D.","family":"Lopaschuk","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Mazankowski Alberta Heart Institute, University of Alberta, Edmonton, Alberta, Canada;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00862.2006"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07181"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1038\/82565"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1172\/JCI36714"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2009.06.010"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.2337\/db09-1471"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1210\/en.2011-1312"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00402.2009"},{"key":"B9","doi-asserted-by":"crossref","first-page":"9812","DOI":"10.1016\/S0021-9258(18)48006-4","volume":"262","author":"Declercq PE","year":"1987","journal-title":"J Biol Chem"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2005.12.021"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2421"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.73.011303.074027"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.2.741"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053142"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00092.2011"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0708385104"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1103267108"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1038\/oby.2006.312"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1526-09.2009"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1038\/nn1626"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1834402100"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.2337\/diab.46.1.150"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.2337\/db08-1039"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2007.07.066"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.53.3.549"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.6.1290"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1102364108"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2005.10.007"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2847"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2005.12.066"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1042\/BST0331063"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1126\/science.288.5475.2379"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1124\/pr.109.002428"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2008.03.006"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.2337\/db05-1356"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1210\/endo.143.2.8652"},{"key":"B38","doi-asserted-by":"crossref","first-page":"4128","DOI":"10.1016\/S0021-9258(17)34693-8","volume":"253","author":"McGarry JD","year":"1978","journal-title":"J Biol Chem"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2008.11.020"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M209999200"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00776.2010"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1038\/nm873"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.51.2.271"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(95)90045-4"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1038\/289401a0"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1006\/geno.2002.6845"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1017\/S0029665112000778"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1042\/bj2060177"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00190.2010"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/69.4.584"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1038\/35007534"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1038\/ijo.2010.159"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M707965200"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00015.2004"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3308-07.2007"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(72)90397-X"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1037\/h0029461"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2010.04.013"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00125.2007"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1990.259.2.E266"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0602205103"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1038\/nn1073"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1038\/nn1336"}],"container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpendo.00168.2013","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,24]],"date-time":"2022-02-24T01:42:50Z","timestamp":1645666970000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpendo.00168.2013"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8,1]]},"references-count":62,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,8,1]]}},"alternative-id":["10.1152\/ajpendo.00168.2013"],"URL":"https:\/\/doi.org\/10.1152\/ajpendo.00168.2013","relation":{},"ISSN":["0193-1849","1522-1555"],"issn-type":[{"value":"0193-1849","type":"print"},{"value":"1522-1555","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8,1]]}}}