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The aim was to examine \u03b2-AR signaling during early regeneration in rat fast-twitch [extensor digitorum longus (EDL)] and slow-twitch (soleus) muscles after bupivacaine injury and test the hypothesis that, during regeneration, \u03b2-agonist administration does not cause \u03b2-AR desensitization. Rats received either the \u03b2-AR agonist fenoterol (1.4 mg\u00b7kg<jats:sup>\u22121<\/jats:sup>\u00b7day<jats:sup>\u22121<\/jats:sup> ip) or saline for 7 days postinjury. Fenoterol reduced \u03b2-AR density in regenerating soleus muscles by 42%. Regenerating EDL muscles showed a threefold increase in \u03b2-AR density, and, again, these values were 43% lower with fenoterol treatment. An amplified adenylate cyclase (AC) response to isoproterenol was observed in cell membrane fragments from EDL and soleus muscles 7 days postinjury. Fenoterol attenuated this increase in regenerating EDL muscles but not soleus muscles. \u03b2-AR signaling mechanisms were assessed using AC stimulants (NaF, forskolin, and Mn<jats:sup>2+<\/jats:sup>). Although \u03b2-agonist treatment reduces \u03b2-AR density in regenerating muscles, these muscles can produce large cAMP responses relative to healthy (uninjured) muscles. Desensitization of \u03b2-AR signaling in regenerating muscles is prevented by altered rates of \u03b2-AR synthesis and\/or degradation, changes in G protein populations and coupling efficiency, and altered AC activity. These mechanisms have important therapeutic implications for modulating \u03b2-AR signaling to enhance muscle repair after injury. <\/jats:p>","DOI":"10.1152\/ajpendo.00175.2007","type":"journal-article","created":{"date-parts":[[2007,7,11]],"date-time":"2007-07-11T02:04:28Z","timestamp":1184119468000},"page":"E932-E940","source":"Crossref","is-referenced-by-count":33,"title":["\u03b2-Adrenoceptor signaling in regenerating skeletal muscle after \u03b2-agonist administration"],"prefix":"10.1152","volume":"293","author":[{"given":"Felice","family":"Beitzel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin N.","family":"Sillence","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon S.","family":"Lynch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.5.9.1650314"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.2001.281.4.R1079"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.2001.281.6.R1895"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00443.2001"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01081.2003"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1378\/chest.109.4.957"},{"key":"R7","doi-asserted-by":"crossref","unstructured":"Burniston JG, Tan LB, Goldspink DF. \u03b22-Adrenergic receptor stimulation in vivo induces apoptosis in rat heart and soleus muscle. 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