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Using C<jats:sub>2<\/jats:sub>C<jats:sub>12<\/jats:sub>myocytes expressing exofacial-Myc-GLUT4-enhanced cyan fluorescent protein, we herein show that differentiated C<jats:sub>2<\/jats:sub>C<jats:sub>12<\/jats:sub>myotubes are equipped with basic GLUT4 translocation machinery that can be activated by insulin stimulation (\u223c3-fold increase as assessed by anti-Myc antibody uptake and immunostaining assay). However, this insulin stimulation of GLUT4 translocation was difficult to demonstrate with a conventional 2-deoxyglucose uptake assay because of markedly elevated basal glucose uptake via other glucose transporter(s). Intriguingly, the basal glucose transport activity in C<jats:sub>2<\/jats:sub>C<jats:sub>12<\/jats:sub>myotubes appeared to be acutely suppressed within 5 min by preincubation with a pathophysiologically high level of extracellular glucose (25 mM). In contrast, this activity was augmented by acute glucose deprivation via an unidentified mechanism that is independent of GLUT4 translocation but is dependent on phosphatidylinositol 3-kinase activity. Taken together, these findings indicate that regulation of the facilitative glucose transport system in differentiated C<jats:sub>2<\/jats:sub>C<jats:sub>12<\/jats:sub>myotubes can be achieved through surprisingly acute glucose-dependent modulation of the activity of glucose transporter(s), which apparently contributes to obscuring the insulin augmentation of glucose uptake elicited by GLUT4 translocation. We herein also describe several methods of monitoring insulin-dependent glucose uptake in C<jats:sub>2<\/jats:sub>C<jats:sub>12<\/jats:sub>myotubes and propose this cell line to be a useful model for analyzing GLUT4 translocation in skeletal muscle.<\/jats:p>","DOI":"10.1152\/ajpendo.00194.2006","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:56:14Z","timestamp":1149065774000},"page":"E817-E828","source":"Crossref","is-referenced-by-count":81,"title":["Regulation of glucose transporters by insulin and extracellular glucose in C<sub>2<\/sub>C<sub>12<\/sub>myotubes"],"prefix":"10.1152","volume":"291","author":[{"given":"Taku","family":"Nedachi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Kanzaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.2000.1935"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.2337\/diab.44.6.695"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1210\/en.2004-1347"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1021\/bi00447a011"},{"key":"R5","doi-asserted-by":"crossref","unstructured":"Charron MJand Kahn BB.Divergent molecular mechanisms for insulin-resistant glucose transport in muscle and adipose cells in vivo.J Biol Chem265: 7994\u20138000, 1990.","DOI":"10.1016\/S0021-9258(19)39029-5"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1002\/cm.20010"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1994.267.6.E808"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.15.3.318"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.2337\/diab.30.12.1000"},{"key":"R10","doi-asserted-by":"crossref","unstructured":"Diamond DLand Carruthers A.Metabolic control of sugar transport by derepression of cell surface glucose transporters. 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