{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:10:13Z","timestamp":1760645413318},"reference-count":60,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:p>The aims of this investigation were 1) to determine whether endurance exercise training could reverse impairments in insulin-stimulated compartmentalization and\/or activation of aPKC\u03b6\/\u03bb and Akt2 in skeletal muscle from high-fat-fed rodents and 2) to assess whether the PPAR\u03b3 agonist rosiglitazone could reverse impairments in skeletal muscle insulin signaling typically observed after high-fat feeding. Sprague-Dawley rats were placed on chow (NORCON, n = 16) or high-fat ( n = 64) diets for 4 wk. During a subsequent 4-wk experimental period, high-fat-fed rats were allocated ( n = 16\/group) to either sedentary control (HFC), exercise training (HFX), rosiglitazone treatment (HFRSG), or a combination of both exercise training and rosiglitazone (HFRX). Following the 4-wk experimental period, animals underwent hindlimb perfusions. Insulin-stimulated plasma membrane-associated aPKC\u03b6 and -\u03bb protein concentration, aPKC\u03b6\/\u03bb activity, GLUT4 protein concentration, cytosolic Akt2, and aPKC\u03b6\/\u03bb activities were reduced ( P &lt; 0.05) in HFC compared with NORCON. Cytosolic Akt2, aPKC\u03b6, and aPKC\u03bb protein concentrations were not affected in HFC compared with NORCON. Exercise training reversed the deleterious effects of the high-fat diet such that insulin-stimulated compartmentalization and activation of components of the insulin-signaling cascade in HFX were normalized to NORCON. High-fat diet-induced impairments to skeletal muscle glucose metabolism were not reversed by rosiglitazone administration, nor did rosiglitazone augment the effect of exercise. Our findings indicate that chronic exercise training, but not rosiglitazone, reverses high-fat diet induced impairments in compartmentalization and activation of components of the insulin-signaling cascade in skeletal muscle.<\/jats:p>","DOI":"10.1152\/ajpendo.00230.2007","type":"journal-article","created":{"date-parts":[[2007,7,11]],"date-time":"2007-07-11T02:04:28Z","timestamp":1184119468000},"page":"E941-E949","source":"Crossref","is-referenced-by-count":28,"title":["Exercise reverses high-fat diet-induced impairments on compartmentalization and activation of components of the insulin-signaling cascade in skeletal muscle"],"prefix":"10.1152","volume":"293","author":[{"suffix":"III","given":"Ben B.","family":"Yaspelkis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah J.","family":"Lessard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald 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