{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:20:59Z","timestamp":1783383659612,"version":"3.54.6"},"reference-count":55,"publisher":"American Physiological Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[2012,11,1]]},"abstract":"<jats:p> Unexpected environmental and social stimuli could trigger stress. Although coping with stress is essential for survival, long-term stress impacts visceral functions, and therefore, it plays a role in the development and exacerbation of symptoms of gastrointestinal\/urogenital disorders. The aim of this study is to characterize the role of corticosterone in stress-sensitized colon-bladder cross-talk, a phenomenon presumed to underlie the comorbidity of functional bowel and bladder disorders. Cystometry and protein\/mRNA expression in the lumbosacral dorsal horn (L6-S1) in response to intracolonic mustard oil (MO) instillation were analyzed in female Wistar-Kyoto rats subjected to water avoidance stress (WAS; 1 h\/day for 10 days) or sham stress (WAsham). Whereas it had no effect on baseline-voiding function, chronic stress upregulated plasma corticosterone concentration and dorsal horn spinal p90 ribosomal S6 kinase 2 (RSK2) protein\/mRNA levels, and RSK2 immunoreactivity colocalized with NeuN-positive neurons. Intracolonic MO dose-dependently decreased intrercontraction intervals and threshold pressure, provoked spinal RSK2 and NR2B phosphorylation, and enhanced PSD-95-RSK2 and PSD-95-NR2B coupling. Intrathecal kaempferol (a RSK2 activation antagonist; 30 min before MO instillation), bilateral adrenalectomy (7 days prior the stress paradigm), and subcutaneous RU-38486 (a glucocorticoid receptor antagonist; 30 min daily before stress sessions), but not RU-28318 (a mineralocorticoid receptor antagonist), attenuated MO-induced bladder hyperactivity, protein phosphorylation, and protein-protein interactions in the WAS group. Our results suggest that stress-associated glucocorticoid release mediates WAS-dependent sensitization of colon-bladder cross-talk via the spinal RSK2\/PSD-95\/NR2B cascade and offer a possibility for developing pharmacological strategies for the treatment of stress-related pelvic pain. <\/jats:p>","DOI":"10.1152\/ajpendo.00235.2012","type":"journal-article","created":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T10:20:26Z","timestamp":1351765226000},"page":"E1094-E1106","source":"Crossref","is-referenced-by-count":11,"title":["Glucocorticoid mediates water avoidance stress-sensitized colon-bladder cross-talk via RSK2\/PSD-95\/NR2B in rats"],"prefix":"10.1152","volume":"303","author":[{"given":"Hsien-Yu","family":"Peng","sequence":"first","affiliation":[{"name":"Department of Medicine, Mackay Medical College, New Taipei, Taiwan;"},{"name":"Department of Urology, China Medical University Hospital, 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