{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T12:13:34Z","timestamp":1790511214030,"version":"4.1.0"},"reference-count":46,"publisher":"American Physiological Society","issue":"1","funder":[{"name":"VU University Amsterdam; Academic medical center Amsterdam","award":["2013-01"],"award-info":[{"award-number":["2013-01"]}]},{"name":"Hercules Foundation","award":["AKUL 13\/47"],"award-info":[{"award-number":["AKUL 13\/47"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[2019,1,1]]},"abstract":"<jats:p> Bone marrow adipose tissue (BMAT) increases after menopause, and increased BMAT is associated with osteoporosis and prevalent vertebral fractures. Peroxisome proliferator-activated receptor-\u03b3 (PPAR\u03b3) activation promotes adipogenesis and inhibits osteoblastogenesis; therefore, PPAR\u03b3 is a potential contributor to the postmenopausal increase in BMAT and decrease in bone mass. The aim of this study is to determine if PPAR\u03b3 inhibition can prevent ovariectomy-induced BMAT increase and bone loss in C3H\/HeJ mice. Fourteen-week-old female C3H\/HeJ mice ( n = 40) were allocated to four intervention groups: sham surgery (Sham) or ovariectomy (OVX; isoflurane anesthesia) with either vehicle (Veh) or PPAR\u03b3 antagonist administration (GW9662; 1 mg\u00b7kg<jats:sup>\u22121<\/jats:sup>\u00b7day<jats:sup>\u22121<\/jats:sup>, daily intraperitoneal injections) for 3 wk. We measured BMAT volume, adipocyte size, adipocyte number. and bone structural parameters in the proximal metaphysis of the tibia using polyoxometalate-based contrast enhanced-nanocomputed topogaphy. Bone turnover was measured in the contralateral tibia using histomorphometry. The effects of surgery and treatment were analyzed by two-way ANOVA. OVX increased the BMAT volume fraction (Sham + Veh: 2.9\u2009\u00b1\u20092.7% vs. OVX + Veh: 8.1\u2009\u00b1\u20095.0%: P &lt; 0.001), average adipocyte diameter (Sham + Veh: 19.3\u2009\u00b1\u20092.6 \u03bcm vs. OVX + Veh: 23.1\u2009\u00b1\u20093.4 \u03bcm: P = 0.001), and adipocyte number (Sham + Veh: 584\u2009\u00b1\u2009337cells\/\u03bcm<jats:sup>3<\/jats:sup> vs. OVX + Veh: 824\u2009\u00b1\u2009113cells\/\u03bcm<jats:sup>3<\/jats:sup>: P = 0.03), while OVX decreased bone volume fraction (Sham + Veh: 15.5\u2009\u00b1\u20092.8% vs. OVX + Veh: 7.7\u2009\u00b1\u20091.9%; P &lt; 0.001). GW9662 had no effect on BMAT, bone structural parameters, or bone turnover. In conclusion, ovariectomy increased BMAT and decreased bone volume in C3H\/HeJ mice. The PPAR\u03b3 antagonist GW9662 had no effect on BMAT or bone volume in C3H\/HeJ mice, suggesting that BMAT accumulation is regulated independently of PPAR\u03b3 in C3H\/HeJ mice. <\/jats:p>","DOI":"10.1152\/ajpendo.00265.2018","type":"journal-article","created":{"date-parts":[[2018,11,20]],"date-time":"2018-11-20T19:59:58Z","timestamp":1542743998000},"page":"E96-E105","source":"Crossref","is-referenced-by-count":17,"title":["The effect of PPAR\u03b3 inhibition on bone marrow adipose tissue and bone in C3H\/HeJ mice"],"prefix":"10.1152","volume":"316","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0713-7096","authenticated-orcid":false,"given":"Kerensa M.","family":"Beekman","sequence":"first","affiliation":[{"name":"Amsterdam Movement Sciences, Section of Endocrinology, Department of Internal Medicine, Vrije Universiteit, Amsterdam University Medical Center, Amsterdam, The Netherlands"},{"name":"Amsterdam Movement Sciences, Department of Radiology and Nuclear Medicine, University 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