{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T12:45:39Z","timestamp":1791117939563,"version":"4.1.0"},"reference-count":36,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:p>Triacylglycerol synthesis in rat epididymal fat overshoots sedentary levels at 10, 29, and 53 h of physical inactivity after 21 days of wheel running. The purposes of the present study were to determine 1) whether this effect is also observed after an acute bout of physical activity and 2) what enzymatic changes might contribute to this effect. We show that more than one bout of physical activity, such as that which occurs with 21 days of wheel running, is necessary for palmitic acid incorporation into triacylglyceride (triglyceride synthesis) to overshoot sedentary values, which suggests that pretranslational mechanisms may be responsible for this overshoot effect. Ten hours after 21 days of wheel running, activity of the mitochondrial glycerol-3-phosphate acyltransferase-1 (mtGPAT1) isoform, a key regulator of triacylglycerol synthesis, overshot sedentary values by 48% and remained higher than sedentary values at 29 and 53 h of reduced physical activity. The overshoot in mtGPAT1 activity was accompanied by an increase in mtGPAT protein level. Cyclic AMP response element-binding protein-binding protein level was higher in sedentary 29 h after 21 days of wheel running. AMP kinase-\u03b1 Thr<jats:sup>172<\/jats:sup>phosphorylation was increased immediately after treadmill running, but decreased to sedentary values by 5 h after activity. Casein kinase-2\u03b1 protein level and activity were unchanged. We conclude that an increase in mtGPAT protein might contribute to the overshoot in triacylglycerol synthesis.<\/jats:p>","DOI":"10.1152\/ajpendo.00321.2005","type":"journal-article","created":{"date-parts":[[2005,10,19]],"date-time":"2005-10-19T03:18:07Z","timestamp":1129691887000},"page":"E480-E489","source":"Crossref","is-referenced-by-count":22,"title":["Increased mitochondrial glycerol-3-phosphate acyltransferase protein and enzyme activity in rat epididymal fat upon cessation of wheel running"],"prefix":"10.1152","volume":"290","author":[{"given":"David S.","family":"Kump","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Laye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank W.","family":"Booth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.49.070180.002331"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.12.6340"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00757.2003"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M309905200"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0214672"},{"key":"R6","doi-asserted-by":"crossref","unstructured":"Coleman Rand Bell RM.Triacylglycerol synthesis in isolated fat cells. Studies on the microsomal diacylglycerol acyltransferase activity using ethanol-dispersed diacylglycerols.J Biol Chem251: 4537\u20134543, 1976.","DOI":"10.1016\/S0021-9258(17)33235-0"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7827(03)00051-1"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/S1095-6433(03)00208-3"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/S1388-1981(00)00140-2"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.28.17865"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.11.7298"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1042\/bst0301091"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M103386200"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.3.1416"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M101661200"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2005.084525"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.073593"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.2001.2604"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M314032200"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(02)02466-X"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1042\/bst0301059"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1042\/bj3380783"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1101\/gad.10.22.2903"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M410422200"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(02)02064-8"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.047233"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1174"},{"key":"R28","doi-asserted-by":"crossref","unstructured":"Schlossman DMand Bell RM.Triacylglycerol synthesis in isolated fat cells. Evidence that the sn-glycerol-3-phosphate and dihydroxyacetone phosphate acyltransferase activities are dual catalytic functions of a single microsomal enzyme.J Biol Chem251: 5738\u20135744, 1976.","DOI":"10.1016\/S0021-9258(17)33118-6"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.19.12536"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1038\/75651"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.nutr.18.1.331"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2335135100"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2002.6341"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.18.8288-8300.2004"},{"key":"R35","unstructured":"US Department of Health and Human Services.Physical Activity and Health: A Report of the Surgeon General.Rockville, MD: US DHHS, Public Health Service, Office of the Surgeon General, 1996."},{"key":"R36","unstructured":"US Department of Health and Human Services.The Surgeon General's Call to Action to Prevent and Decrease Overweight and Obesity.Rockville, MD: US DHHS, Public Health Service, Office of the Surgeon General, 2001."}],"container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpendo.00321.2005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,18]],"date-time":"2021-07-18T17:33:15Z","timestamp":1626629595000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpendo.00321.2005"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,3]]},"references-count":36,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,3]]}},"alternative-id":["10.1152\/ajpendo.00321.2005"],"URL":"https:\/\/doi.org\/10.1152\/ajpendo.00321.2005","relation":{},"ISSN":["0193-1849","1522-1555"],"issn-type":[{"value":"0193-1849","type":"print"},{"value":"1522-1555","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,3]]}}}