{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:57:44Z","timestamp":1790711864214,"version":"4.1.0"},"reference-count":49,"publisher":"American Physiological Society","issue":"2","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China (NSFC)","doi-asserted-by":"publisher","award":["81670344"],"award-info":[{"award-number":["81670344"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China (NSFC)","doi-asserted-by":"publisher","award":["81570340"],"award-info":[{"award-number":["81570340"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Graduate innovation Foundation of Harbin Medical University","award":["YJSCX2017-7HYD"],"award-info":[{"award-number":["YJSCX2017-7HYD"]}]},{"name":"Educatiion department of Heilongjiang Provious","award":["12531382"],"award-info":[{"award-number":["12531382"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[2019,8,1]]},"abstract":"<jats:p> Hydrogen sulfide (H<jats:sub>2<\/jats:sub>S), a gaseous molecule, is involved in modulating multiple physiological functions, such as antioxidant, antihypertension, and the production of polysulfide cysteine. H<jats:sub>2<\/jats:sub>S may inhibit reactive oxygen species generation and ATP production through modulating respiratory chain enzyme activities; however, the mechanism of this effect remains unclear. In this study, db\/db mice, neonatal rat cardiomyocytes, and H9c2 cells treated with high glucose, oleate, and palmitate were used as animal and cellular models of type 2 diabetes. The mitochondrial respiratory rate, respiratory chain complex activities, and ATP production were decreased in db\/db mice compared with those in db\/db mice treated with exogenous H<jats:sub>2<\/jats:sub>S. Liquid chromatography with tandem mass spectrometry analysis showed that the acetylation level of proteins involved in the mitochondrial respiratory chain were increased in the db\/db mice hearts compared with those with sodium hydrosulfide (NaHS) treatment. Exogenous H<jats:sub>2<\/jats:sub>S restored the ratio of NAD<jats:sup>+<\/jats:sup>\/NADH, enhanced the expression and activity of sirtuin 3 (SIRT3) and decreased mitochondrial acetylation level in cardiomyocytes under hyperglycemia and hyperlipidemia. As a result of SIRT3 activation, acetylation of the respiratory complexe enzymes NADH dehydrogenase 1 (ND1), ubiquinol cytochrome c reductase core protein 1, and ATP synthase mitochondrial F1 complex assembly factor 1 was reduced, which enhanced the activities of the mitochondrial respiratory chain activity and ATP production. We conclude that exogenous H<jats:sub>2<\/jats:sub>S plays a critical role in improving cardiac mitochondrial function in diabetes by upregulating SIRT3. <\/jats:p>","DOI":"10.1152\/ajpendo.00326.2018","type":"journal-article","created":{"date-parts":[[2019,6,11]],"date-time":"2019-06-11T15:20:27Z","timestamp":1560266427000},"page":"E284-E297","source":"Crossref","is-referenced-by-count":45,"title":["Exogenous H<sub>2<\/sub>S reduces the acetylation levels of mitochondrial respiratory enzymes via regulating the NAD<sup>+<\/sup>-SIRT3 pathway in cardiac tissues of <i>db\/db<\/i> mice"],"prefix":"10.1152","volume":"317","author":[{"given":"Yu","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Pathophysiology, Harbin Medical University, Harbin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongyan","family":"Teng","sequence":"additional","affiliation":[{"name":"Department of Geriatrics, Second Affiliated Hospital of Harbin Medical University, Harbin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojiao","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Harbin Medical University, Harbin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linxue","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Harbin Medical University, Harbin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Harbin Medical University, Harbin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingzhu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Harbin Medical University, Harbin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangping","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Harbin Medical University, Harbin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Harbin Medical University, Harbin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Harbin Medical University, Harbin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Harbin Medical University, Harbin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urologic Surgery, First Affiliated Hospital of Harbin Medical University, Harbin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dechao","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Cardiology, First affiliated hospital of Harbin Medical University, Harbin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajun","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Harbin Medical University, Harbin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Immunology, Harbin Medical University, Harbin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongyi","family":"Cheng","sequence":"additional","affiliation":[{"name":"Jingjie PTM BioLab, Co., Ltd. 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