{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T14:38:41Z","timestamp":1793198321489,"version":"4.5.0"},"reference-count":40,"publisher":"American Physiological Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[2015,12,1]]},"abstract":"<jats:p>\u03b2-Thalassemia, a hereditary anemic disorder, is often associated with skeletal complications that can be found in both males and females. The present study aimed to investigate the age- and sex-dependent changes in bone mineral density (BMD) and trabecular microstructure in \u03b2<jats:sup>IVSII-654<\/jats:sup>knockin thalassemic mice. Dual-energy X-ray absorptiometry and computer-assisted bone histomorphometry were employed to investigate temporal changes in BMD and histomorphometric parameters in male and female mice of a \u03b2<jats:sup>IVSII-654<\/jats:sup>knockin mouse model of human \u03b2-thalassemia, in which impaired splicing of \u03b2-globin transcript was caused by hemizygous C\u2192T mutation at nucleotide 654 of intron 2. Young, growing \u03b2<jats:sup>IVSII-654<\/jats:sup>mice (1 mo old) manifested shorter bone length and lower BMD than their wild-type littermates, indicating possible growth retardation and osteopenia, the latter of which persisted until 8 mo of age (adult mice). Interestingly, two-way analysis of variance suggested an interaction between sex and \u03b2<jats:sup>IVSII-654<\/jats:sup>genotype, i.e., more severe osteopenia in adult female mice. Bone histomorphometry further suggested that low trabecular bone volume in male \u03b2<jats:sup>IVSII-654<\/jats:sup>mice, particularly during a growing period (1\u20132 mo), was primarily due to suppression of bone formation, whereas both a low bone formation rate and a marked increase in osteoclast surface were observed in female \u03b2<jats:sup>IVSII-654<\/jats:sup>mice. In conclusion, osteopenia and trabecular microstructural defects were present in both male and female \u03b2<jats:sup>IVSII-654<\/jats:sup>knockin thalassemic mice, but the severity, disease progression, and cellular mechanism differed between the sexes.<\/jats:p>","DOI":"10.1152\/ajpendo.00329.2015","type":"journal-article","created":{"date-parts":[[2015,10,21]],"date-time":"2015-10-21T17:12:45Z","timestamp":1445447565000},"page":"E936-E948","source":"Crossref","is-referenced-by-count":14,"title":["Bone microstructural defects and osteopenia in hemizygous \u03b2<sup>IVSII-654<\/sup>knockin thalassemic mice: sex-dependent changes in bone density and osteoclast function"],"prefix":"10.1152","volume":"309","author":[{"given":"Kanogwun","family":"Thongchote","sequence":"first","affiliation":[{"name":"Center of Calcium and Bone Research, Faculty of Science, Mahidol University, Bangkok, Thailand;"},{"name":"Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand;"},{"name":"Exercise Science Graduate Program, Faculty of Science, Mahidol University, Bangkok, Thailand; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saovaros","family":"Svasti","sequence":"additional","affiliation":[{"name":"Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jarinthorn","family":"Teerapornpuntakit","sequence":"additional","affiliation":[{"name":"Center of Calcium and Bone Research, Faculty of Science, Mahidol University, Bangkok, Thailand;"},{"name":"Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panan","family":"Suntornsaratoon","sequence":"additional","affiliation":[{"name":"Center of Calcium and Bone Research, Faculty of Science, Mahidol University, Bangkok, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nateetip","family":"Krishnamra","sequence":"additional","affiliation":[{"name":"Center of Calcium and Bone Research, Faculty of Science, Mahidol University, Bangkok, Thailand;"},{"name":"Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narattaphol","family":"Charoenphandhu","sequence":"additional","affiliation":[{"name":"Center of Calcium and Bone Research, Faculty of Science, Mahidol University, Bangkok, Thailand;"},{"name":"Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1982.00340170191029"},{"key":"B2","first-page":"E769","volume":"236","author":"Armbrecht HJ","year":"1979","journal-title":"Am J Physiol Endocrinol Metab Gastrointest Physiol"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M409332200"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2005.02.007"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.2956"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.9.2821"},{"key":"B7","first-page":"72","author":"De Sanctis V","year":"2002","journal-title":"Horm Res 58"},{"key":"B8","first-page":"643","volume":"72","author":"De Sanctis V","year":"1988","journal-title":"Obstet Gynecol"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.1805"},{"key":"B10","first-page":"1425","volume":"28","author":"Dundar U","year":"2007","journal-title":"Saudi Med J"},{"key":"B11","unstructured":"Eriksen E, Axelrod D, Melsen F.Bone Histomorphometry. New York: Raven, 1994, p. 33\u201350."},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-09-048868"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2001.24050"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2010.10.173"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.4.489"},{"key":"B16","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1182\/blood.V72.4.1107.1107","volume":"72","author":"Kazazian HH","year":"1988","journal-title":"Blood"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2003.10.005"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116383"},{"key":"B19","doi-asserted-by":"crossref","first-page":"2152","DOI":"10.1182\/blood.V91.6.2152","volume":"91","author":"Lewis J","year":"1998","journal-title":"Blood"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2013.12.003"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1624938"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1159\/000321189"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1997.d01-962.x"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1111\/iep.12044"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/134.8.1857"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.005827"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00556.2013"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00134.2010"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20041650"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-011-0823-1"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00111.2014"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1136\/adc.53.4.347"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2010.08.003"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1998.tb10491.x"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2009.05.021"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1089\/107999099314243"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2196"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1210\/endo-123-2-681"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/109.4.573"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20111566"}],"container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpendo.00329.2015","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,10]],"date-time":"2020-09-10T01:39:45Z","timestamp":1599701985000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpendo.00329.2015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12,1]]},"references-count":40,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2015,12,1]]}},"alternative-id":["10.1152\/ajpendo.00329.2015"],"URL":"https:\/\/doi.org\/10.1152\/ajpendo.00329.2015","relation":{},"ISSN":["0193-1849","1522-1555"],"issn-type":[{"value":"0193-1849","type":"print"},{"value":"1522-1555","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,12,1]]}}}