{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T00:25:12Z","timestamp":1793060712471,"version":"4.5.0"},"reference-count":53,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:p> Hyperandrogenism and chronic low-grade inflammation are related in polycystic ovary syndrome (PCOS), but it is unknown whether hyperandrogenemia can activate inflammation. We determined the effect of oral androgen administration on fasting and glucose-stimulated nuclear factor-\u03baB (NF-\u03baB) activation and expression and related markers of inflammation in mononuclear cells (MNC) of lean reproductive-age women. Sixteen lean, ovulatory reproductive-age women were treated with 130 mg of DHEA or placebo ( n = 8 each) for 5 days in a randomized, controlled, double-blind fashion. Nuclear activation of NF-\u03baB, p65 and p105 NF-\u03baB subunit RNA, TNF\u03b1 and IL-1\u03b2 mRNA, and NF-\u03baB p65 and inhibitory-\u03baB (I\u03baB) protein were quantified from MNC obtained while fasting and 2 h after glucose ingestion, before and after DHEA or placebo administration. Before treatment, subjects receiving DHEA or placebo exhibited no differences in androgens or any inflammatory markers while fasting and after glucose ingestion. Compared with placebo, DHEA administration raised levels of testosterone, androstenedione, and DHEA-S, increased the percent change in fasting and glucose-challenged activated NF-\u03baB, p65, p105, TNF\u03b1, and IL-1\u03b2 RNA and p65 protein, and decreased the percent change in fasting and glucose-challenged I\u03baB protein. 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