{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T15:30:44Z","timestamp":1757777444906},"reference-count":29,"publisher":"American Physiological Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[2014,6,15]]},"abstract":"<jats:p>We have reported an early decrease in glycemia in rats fed a biotin-deficient diet with reduced cellular ATP levels, suggesting increased insulin sensitivity. Here, we show that biotin-deprived rats are more tolerant of glucose, as shown by both oral and intraperitoneal glucose tolerance tests, during which insulin plasma levels were significantly diminished in deficient rats compared with controls. Biotin-deficient rats had lower blood glucose concentrations during intraperitoneal insulin sensitivity tests than controls. Furthermore, more glucose was infused to maintain euglycemia in the biotin-deficient rats during hyperinsulinemic euglycemic clamp studies. These results demonstrate augmented sensitivity to insulin in biotin-deprived rats. They are most likely the consequence of an insulin-independent effect of AMPK activation on GLUT4 membrane translocation with increased glucose uptake. In biotin-deficient cultured L6 muscle cells, there was increased phosphorylation of the energy sensor AMPK. We have now confirmed the augmented AMPK activation in both biotin-deprived in vivo muscle and cultured muscle cells. In these cells, glucose uptake is increased by AMPK activation by AICAR and diminished by its knockdown by the specific siRNAs directed against its \u03b11- and \u03b12-catalytic subunits, with all of these effects being largely independent of the activity of the insulin-signaling pathway that was inhibited with wortmannin. The enhanced insulin sensitivity in biotin deficiency likely has adaptive value for organisms due to the hormone promotion of uptake and utilization of not only glucose but other nutrients such as branched-chain amino acids, whose deficiency has been reported to increase insulin tolerance.<\/jats:p>","DOI":"10.1152\/ajpendo.00442.2013","type":"journal-article","created":{"date-parts":[[2014,5,7]],"date-time":"2014-05-07T06:57:25Z","timestamp":1399445845000},"page":"E1442-E1448","source":"Crossref","is-referenced-by-count":4,"title":["Insulin sensitivity is inversely related to cellular energy status, as revealed by biotin deprivation"],"prefix":"10.1152","volume":"306","author":[{"given":"Ana","family":"Salvador-Adriano","sequence":"first","affiliation":[{"name":"Unidad de Gen\u00e9tica de la Nutrici\u00f3n, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico and Instituto Nacional de Pediatr\u00eda, Mexico City, Mexico;"},{"name":"Instituto Nacional de Pediatr\u00eda, Mexico City, Mexico;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia","family":"Vargas-Ch\u00e1vez","sequence":"additional","affiliation":[{"name":"Unidad de Gen\u00e9tica de la Nutrici\u00f3n, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico and Instituto Nacional de Pediatr\u00eda, Mexico City, Mexico;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alain de J.","family":"Hern\u00e1ndez-V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Unidad de Gen\u00e9tica de la Nutrici\u00f3n, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico and Instituto Nacional de Pediatr\u00eda, Mexico City, Mexico;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Ortega-Cuellar","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Pediatr\u00eda, Mexico City, Mexico;"},{"name":"Laboratorio de Nutrici\u00f3n Experimental, Mexico City, Mexico; 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