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Gastrocnemius muscle from male Sprague-Dawley rats trained to consume a meal consisting of rat chow was sampled before, during, and after the meal. Meal feeding enhanced the assembly of the active eIF4G\u00b7eIF4E complex, which returned to basal levels within 3 h of removal of food. The increased assembly of the active eIF4G\u00b7eIF4E complex was associated with a marked 10-fold rise in phosphorylation of eIF4G(Ser<jats:sup>1108<\/jats:sup>) and a decreased assembly of inactive 4E-BP1\u00b7eIF4E complex. The reduced assembly of 4E-BP1\u00b7eIF4E complex was associated with a 75-fold increase in phosphorylation of 4E-BP1 in the \u03b3-form during feeding. Phosphorylation of S6K1 on Ser<jats:sup>789<\/jats:sup>was increased by meal feeding, although the extent of phosphorylation was greater at 0.5 h after feeding than after 1 h. Phosphorylation of mammalian target of rapamycin (mTOR) on Ser<jats:sup>2448<\/jats:sup>or Ser<jats:sup>2481<\/jats:sup>, an upstream kinase responsible for phosphorylating both S6K1 and 4E-BP1, was increased at all times during meal feeding, although the extent of phosphorylation was greater at 0.5 h after feeding than after 1 h. Phosphorylation of PKB, an upstream kinase responsible for phosphorylating mTOR, was elevated only after 0.5 h of meal feeding for Thr<jats:sup>308<\/jats:sup>, whereas phosphorylation Ser<jats:sup>473<\/jats:sup>was significantly elevated at only 0.5 and 1 h after initiation of feeding. We conclude from these studies that meal feeding stimulates two signal pathways in skeletal muscle that lead to elevated eIF4G\u00b7eIF4E complex assembly through increased phosphorylation of eIF4G and decreased association of 4E-BP1 with eIF4E.<\/jats:p>","DOI":"10.1152\/ajpendo.00460.2005","type":"journal-article","created":{"date-parts":[[2005,11,2]],"date-time":"2005-11-02T01:23:56Z","timestamp":1130894636000},"page":"E631-E642","source":"Crossref","is-referenced-by-count":46,"title":["Meal feeding enhances formation of eIF4F in skeletal muscle: role of increased eIF4E availability and eIF4G phosphorylation"],"prefix":"10.1152","volume":"290","author":[{"given":"Thomas C.","family":"Vary","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J.","family":"Lynch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(06)00122-9"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/131.3.856S"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/130.2.139"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00208.2001"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/130.10.2413"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006823109415"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.2001.281.3.E565"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/134.7.1704"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1038\/377441a0"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/135.3.376"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M205838200"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(95)02233-3"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.3109\/10409239409083485"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(03)00220-X"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.11.1422"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1101\/gad.887201"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb00257.x"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.23.14484"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1038\/ncb839"},{"key":"R20","unstructured":"Jefferson LS, Vary TC, and Kimball SR.Regulation of protein metabolism in muscle. 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