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Between 80 and 210 days of age, gene expressions and\/or activities of enzymes involved in lipogenesis or lipolysis were much lower ( P &lt; 0.05) in adipocytes isolated from muscle than in those from other locations. Insulin-induced lipogenesis and lipolytic efficiency after catecholamine addition were also the lowest ( P &lt; 0.05) in IM adipocytes. In these cells, the age-related increase (+300%) in the ratio of mRNA levels of fatty acid synthase to hormone-sensitive lipase paralleled the enlargement of adipocyte diameters (+70%, P &lt; 0.05) and the increase in lipid content in muscle (+135%, P &lt; 0.05) during growth. Expressions of genes coding for leptin, adiponectin, and IGF-I, as well as for various hormonal receptors, were lower ( P &lt; 0.05) in IM adipocytes than in other adipocytes, whereas levels of TNF-\u03b1 mRNA did not differ between sites. Interestingly, IGF-II mRNA levels were higher ( P &lt; 0.05) in IM adipocytes than in other adipocytes. 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