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Control ( db<jats:sup>+<\/jats:sup>) and genetically diabetic ( db\/db) C57BL\/KsJ-lepr<jats:sup>db<\/jats:sup>\/lepr<jats:sup>db<\/jats:sup>mice were subjected to sedentary or treadmill exercise regimens. Exercise training consisted of high-intensity\/short-duration (10 days) and low-intensity\/long-duration (6 wk) protocols. Glycogen levels were elevated by 35\u201350% in db\/db hearts . Exercise training further increased (2- to 3-fold) glycogen levels in db\/db hearts. Analysis of soluble and insoluble glycogen pools revealed no differential accumulation of one glycogen subspecies. Phosphorylation (Ser<jats:sup>640<\/jats:sup>) of glycogen synthase, an indicator of enzymatic fractional activity, was greater in db\/db mice subjected to sedentary and exercise regimens. Elevated glycogen levels were accompanied by decreased phosphorylation (Thr<jats:sup>172<\/jats:sup>) of 5\u2032-AMP-activated kinase and phosphorylation (Ser<jats:sup>79<\/jats:sup>) of its downstream substrate acetyl-CoA carboxylase. Glycogen concentration was not associated with increases in other glycogen-associated proteins, including malin and laforin. Novel observations show that exercise training does not correct diabetes-induced elevations in cardiac glycogen but, rather, precipitates further accumulation.<\/jats:p>","DOI":"10.1152\/ajpendo.00525.2010","type":"journal-article","created":{"date-parts":[[2011,3,9]],"date-time":"2011-03-09T04:48:05Z","timestamp":1299646085000},"page":"E31-E39","source":"Crossref","is-referenced-by-count":27,"title":["Exercise training does not correct abnormal cardiac glycogen accumulation in the<i>db\/db<\/i>mouse model of type 2 diabetes"],"prefix":"10.1152","volume":"301","author":[{"given":"Jane","family":"Shearer","sequence":"first","affiliation":[{"name":"Faculty of Kinesiology and"},{"name":"Departments of 2Biochemistry and Molecular Biology and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen D.","family":"Ross","sequence":"additional","affiliation":[{"name":"Physiology and Pharmacology, Faculty of Medicine, University of Calgary, Calgary, Alberta, 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